跳转至内容

很抱歉,目前系统维护中。在此期间,网站下单功能无法使用。给您带来不便,深表歉意。如需其他帮助,请联系客户联络中心:400 620 3333。

Merck
CN
  • Progressive Rotavirus Infection Downregulates Redox-Sensitive Transcription Factor Nrf2 and Nrf2-Driven Transcription Units.

Progressive Rotavirus Infection Downregulates Redox-Sensitive Transcription Factor Nrf2 and Nrf2-Driven Transcription Units.

Oxidative medicine and cellular longevity (2020-04-24)
Upayan Patra, Urbi Mukhopadhyay, Arpita Mukherjee, Rakesh Sarkar, Mamta Chawla-Sarkar
摘要

Eukaryotic cells adopt highly tuned stress response physiology under threats of exogenous stressors including viruses to maintain cellular homeostasis. Not surprisingly, avoidance of cellular stress response pathways is an essential facet of virus-induced obligatory host reprogramming to invoke a cellular environment conducive to viral perpetuation. Adaptive cellular responses to oxidative and electrophilic stress are usually taken care of by an antioxidant defense system, core to which lies the redox-responsive transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) and Nrf2-driven transcriptional cascade. Deregulation of host redox balance and redox stress-sensitive Nrf2 antioxidant defense have been reported for many viruses. In the current study, we aimed to study the modulation of the Nrf2-based host cellular redox defense system in response to Rotavirus (RV) infection in vitro. Interestingly, we found that Nrf2 protein levels decline sharply with progression of RV infection beyond an initial upsurge. Moreover, Nrf2 decrease as a whole was found to be accompanied by active nuclear vacuity of Nrf2, resulting in lowered expression of stress-responsive Nrf2 target genes heme oxygenase-1 (HO-1), NAD(P)H quinone dehydrogenase 1, and superoxide dismutase 1 both in the presence and absence of Nrf2-driven transcriptional inducers. Initial induction of Nrf2 concurred with RV-induced early burst of oxidative stress and therefore was sensitive to treatments with antioxidants. Reduction of Nrf2 levels beyond initial hours, however, was found to be independent of the cellular redox status. Furthermore, increasing the half-life of Nrf2 through inhibition of the Kelch-like erythroid cell-derived protein with CNC homology- (ECH-) associated protein 1/Cullin3-RING Box1-based canonical Nrf2 turnover pathway could not restore Nrf2 levels post RV-SA11 infection. Depletion of the Nrf2/HO-1 axis was subsequently found to be sensitive to proteasome inhibition with concurrent observation of increased K48-linked ubiquitination associated with Nrf2. Together, the present study describes robust downregulation of Nrf2-dependent cellular redox defense beyond initial hours of RV infection, justifying our previous observation of potent antirotaviral implications of Nrf2 agonists.

材料
货号
品牌
产品描述

Sigma-Aldrich
单克隆抗-FLAG® M2 小鼠抗, 1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
磷酸酶抑制剂混合物2, aqueous solution (dark coloration may develop upon storage, which does not affect the activity)
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
PMA, for use in molecular biology applications, ≥99% (HPLC)
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
噻唑蓝, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
2',7'-二氯荧光素二乙酸酯, ≥97%
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
蛋白酶抑制剂混合物粉末, for general use, lyophilized powder
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
MG-132, A cell-permeable, potent, reversible proteasome inhibitor (Ki = 4 nM).
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
聚肌苷酸-聚胞苷酸 钠盐, TLR ligand tested
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
氯化高铁血红素, from bovine, ≥90%
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
吡咯烷二硫代氨基甲酸铵, ~99%
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
叔丁基氢醌, 97%
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
二亚苯基碘鎓氯化物, ≥98%
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
巴弗洛霉素A1
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
LY-294,002 盐酸盐, solid, ≥98% (HPLC)
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
SB 203580, solid, ≥98% (HPLC)
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
N -乙基马来酰亚胺, crystalline, ≥98% (HPLC)
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
N-乙酰基-L-半胱氨酸, Sigma Grade, ≥99% (TLC), powder
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
CDDO 甲酯, ≥98% (HPLC)
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
Gö 6983, ≥97%
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
鸦胆子苦醇, ≥95% (HPLC)
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
PKR 抑制剂, The PKR Inhibitor, also referenced under CAS 608512-97-6, controls the biological activity of PKR. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21
Sigma-Aldrich
Amyloid Protein Non-Aβ Component, ≥80% (HPLC)
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
请联系客服了解存货情况
¥7,564.21