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D6883

Sigma-Aldrich

2',7'-二氯荧光素二乙酸酯

≥97% (HPLC), powder, cell-permeable non-fluorescent probe

别名:

DCFH-DA, 2′,7′-二氯二氢荧光素二乙酸酯

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About This Item

经验公式（希尔记法）:
C24H16Cl2O7
CAS号:
4091-99-0
分子量:
487.29
Beilstein:
4610273
MDL编号:
MFCD00128955
UNSPSC代码:
12352200
PubChem化学物质编号:
24894058
NACRES:
NA.77

产品名称

2',7'-二氯荧光素二乙酸酯, ≥97%

质量水平

200

方案

≥97%

表单

powder

储存温度

−20°C

SMILES字符串

CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C(c2cc1Cl)c4ccccc4C(O)=O

InChI

1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)

InChI key

PXEZTIWVRVSYOK-UHFFFAOYSA-N

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一般描述

2′,7′-二氯二氢荧光素二乙酸酯（DCFH-DA）是一种对氧化敏感的荧光染料，用于检测细胞内活性氧（ROS）水平。DCFH-DA是一种细胞渗透性非荧光探针，可被细胞内酯酶水解为DCFH，当被细胞内的ROS氧化时形成荧光DCF。
The excitation wavelength is 504 nm and emission wavelength is 529 nm in ethanol. The use of DMSO as the solvent has not been determined, however various publications suggest that it is suitable.

应用

2′,7′-二氯荧光素二乙酸酯适用于：
  • 敏感并快速定量活性氧，以响应氧化代谢
  • 微孔板法，以检测吞噬细胞中的氧化产物
  • 定量多孔髓样分化测定

适用于：敏感并快速定量活性氧，以响应氧化代谢；微孔板法，以检测吞噬细胞中的氧化产物；髓样分化定量多孔测定。

储存及稳定性

本品应在-20℃下以粉末形式保存。

其他说明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

储存分类代码

11 - Combustible Solids

WGK

WGK 3

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000400210
0000352514
0000341644
0000335437
0000352514

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How should Product D6883, 2´,7´-Dichlorofluorescin diacetate, be dissolved?

    2´,7´-Dichlorofluorescin diacetate is soluble in organic solvents such as DMSO, ethanol, and DMF at 25 mg/mL. We test the solubility at 10 mg/ml in methanol.

  4. What is the solution stability of Product D6883, 2´,7´-Dichlorofluorescin diacetate?

    This product can be stored at -20°C for up to six months in solvents such as DMSO, ethanol, or DMF (N,N-dimethylformamide).

  5. What is the excitation and emission wavelength for Product D6883, 2´,7´-Dichlorofluorescin diacetate?

    The excitation wavelength is 504 nm and emission wavelength is 529 nm (in ethanol).

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Life sciences, 232, 116610-116610 (2019-06-30)
The aim of this study was the characterization of the in vitro cytotoxic properties of a recently isolated diterpene compound, 7β-acetoxy-20-hydroxy-19,20-epoxyroyleanone (compound 1), extracted from Salvia corrugata, versus human cell lines. We used as model study immortalized breast epithelial cells
Pornthip Chaichompoo et al.
Journal of clinical pathology, 72(8), 520-524 (2019-04-24)
Iron overload is a major factor contributing to the overall pathology of thalassaemia, which is primarily mediated by ineffective erythropoiesis and shorter mature red blood cell (RBC) survival. Iron accumulation in RBCs generates reactive oxygen species (ROS) that cause cellular
Yuan Ji et al.
Journal of agricultural and food chemistry, 67(32), 9060-9069 (2019-07-25)
Glutathione S-transferases (GSTs) play an active role in the development of drug resistance by numerous cancer cells, including melanoma cells, which is a major cause of chemotherapy failure. As part of our continuous effort to explore why dietary polyphenols bearing
Yeonhwa Song et al.
Journal of experimental & clinical cancer research : CR, 36(1), 38-38 (2017-03-04)
CD133-positive cells in hepatocellular carcinoma (HCC) exhibit cancer stem cell (CSC)-like properties as well as resistance to chemotherapeutic agents and ionizing radiation; however, their function remains unknown. In this paper, we identified a hitherto unknown mechanism to overcome CD133-induced resistance
Shengyan Wang et al.
Electrophoresis, 40(16-17), 2204-2210 (2019-04-07)
Nanoagents achieving photodynamic therapy (PDT) and photothermal therapy (PTT) combination treatment with improved therapeutic effect are highly desirable. However, the incorporation of both PDT and PTT into a single nanoagent often requires multistep fabrication process. Herein, we report that photoactive
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