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Merck
CN

D6883

Sigma-Aldrich

2',7'-二氯荧光素二乙酸酯

≥97% (HPLC), powder, cell-permeable non-fluorescent probe

别名:

2′,7′-二氯二氢荧光素二乙酸酯

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About This Item

经验公式(希尔记法):
C24H16Cl2O7
CAS号:
分子量:
487.29
Beilstein:
4610273
MDL编号:
UNSPSC代码:
12352200
PubChem化学物质编号:
NACRES:
NA.77

product name

2',7'-二氯荧光素二乙酸酯, ≥97%

质量水平

检测方案

≥97%

形式

powder

储存温度

−20°C

SMILES字符串

CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C(c2cc1Cl)c4ccccc4C(O)=O

InChI

1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)

InChI key

PXEZTIWVRVSYOK-UHFFFAOYSA-N

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一般描述

2′,7′-二氯荧光素二乙酸酯是一种细胞可渗透非荧光探针。它可在细胞内脱酯化并经氧化转化为具有强荧光性的2′,7′-二氯荧光素。

应用

2′,7′-二氯荧光素二乙酸酯适用于:
  • 敏感并快速定量活性氧,以响应氧化代谢
  • 微孔板法,以检测吞噬细胞中的氧化产物
  • 定量多孔髓样分化测定

适用于:敏感并快速定量活性氧,以响应氧化代谢;微孔板法,以检测吞噬细胞中的氧化产物;髓样分化定量多孔测定。

储存及稳定性

本品应在-20℃下以粉末形式保存。

WGK

WGK 3

个人防护装备

Eyeshields, Gloves, type N95 (US)


分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How should Product D6883, 2´,7´-Dichlorofluorescin diacetate, be dissolved?

    2´,7´-Dichlorofluorescin diacetate is soluble in organic solvents such as DMSO, ethanol, and DMF at 25 mg/mL. We test the solubility at 10 mg/ml in methanol.

  4. What is the solution stability of Product D6883, 2´,7´-Dichlorofluorescin diacetate?

    This product can be stored at -20°C for up to six months in solvents such as DMSO, ethanol, or DMF (N,N-dimethylformamide).

  5. What is the excitation and emission wavelength for Product D6883, 2´,7´-Dichlorofluorescin diacetate?

    The excitation wavelength is 504 nm and emission wavelength is 529 nm (in ethanol).

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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商品

氧化应激,在一部分上,是由多种细胞过程产生的活性氧介导,并由诸如酶清除剂或抗氧化剂调节剂等细胞抗氧化剂机制所控制的。自由基,如活性氧,会通过细胞引起细胞损伤。

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

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