product name
蛋白酶抑制剂混合物粉末, for general use, lyophilized powder
生物来源
bovine lung
质量水平
形式
lyophilized powder
储存温度
−20°C
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相关类别
一般描述
特异性
应用
- 加入放射性免疫沉淀分析缓冲液，以裂解禽类晶状体样本用于蛋白质免疫沉淀分析
- 加入磷酸缓冲液(PBS)以研磨已表达重组蛋白的叶子，再纯化其中的病毒样颗粒(VLP)
- 加入PBS，以溶解棕点湍蛙皮肤分泌物的冻干样本以便纯化多肽
- 加入0.1 M NaCl溶液，用于洗涤和收集青蛙的皮肤分泌物
生化/生理作用
组分
AEBSF，2 mM
抑肽酶(Aprotinin)，0.3 μM
抑氨肽素(Bestatin)，116 μ
E-64，14 μM
EDTA，1 mM
亮抑酶肽(Leupeptin)，1 μM
每种组分各具特定的抑制特性。
注意
数量
并非所有提取物都含有相同水平的内源酶，并且可能需要调整所需的混合液用量。
制备说明
其他说明
相关产品
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
How much of Product P2714, Protease Inhibitor Cocktail, needs to be used in cell lysates?
One bottle is recommended for the inhibition of proteases present in a maximum of 20 grams of cell extract.
How should I dissolve and store Product P2714, Protease Inhibitor Cocktail?
You can dissolve the product in 10 ml of water to make a 10X stock solution and then dilute 1:10 for use. Aliquots can be stored at -20 C for 1 month.
How much of Product P2714, Protease Inhibitor Cocktail, should I use with serum?
Dilute the contents of the vial in 10 mL of water, yielding a 10X initial concentration . Then dilute the stock 1:10 in the serum (1 part of inhibitor solution to 10 parts of serum). This would be a 1:100 dilution (containing the concentrations of each inhibitor as listed on the data sheet) in the serum. This is true for most cell lysates, serum, and culture media.
Can Product P2714, Protease Inhibitor Cocktail, be used with ELISA?
Yes, because it is water based.
Can Product P2714, Protease Inhibitor Cocktail,be used with Nickel columns?
No because it contains EDTA.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the difference between Protease Inhibitors S8820 and P2714?
S8820 is the tablet version of P2714.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
相关内容
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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