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Dear Customer:

The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

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主要文件

796-0658

HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2)

application for HPLC

别名:

Proteins, mixture

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1 KIT
¥5,713.28

¥5,713.28

国内现货，预计发货时间2025年4月27日详情

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1 KIT
¥5,713.28

About This Item

¥5,713.28

国内现货，预计发货时间2025年4月27日详情

描述

Separated on Discovery® BIO PolyMA-SCX

技术

HPLC: suitable

试验参数

column: Discovery BIO PolyMA-SCX, 5 cm x 4.6 mm, 5 μm particles (59601-U)
mobile phase: [A] 20 mM sodium phosphate, pH 7.0; [B] 20 mM sodium phosphate, 0.5 M NaCl, pH 7.0; 5 to 100% B in 15 min. (linear)
flow rate: 0.5 mL/min
injection: 10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A
detector: UV, 280 nm

适用性

application for HPLC

应用

life science and biopharma

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Mouse IL-15 ELISA Kit for serum, plasma and cell culture supernatant

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Gene Information

mouse ... Il10(16153)

Gene Information

rat ... Il10(25325)

Gene Information

mouse ... Il11(16156)

Gene Information

mouse ... Il15(16168)

species reactivity

mouse

species reactivity

rat

species reactivity

mouse

species reactivity

mouse

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 45 pg/mL
standard curve range: 78.13-5000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 8.23-6000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1000 pg/mL
standard curve range: 1.85-450 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 500 pg/mL
standard curve range: 0.33-80 ng/mL

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

分析说明

The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the power of ion-exchange to resolve proteins with very little difference in molecular weight, an advantage over size-exclusion separations.

法律信息

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

标准

产品编号
说明
价格

C4011

细胞色素 C 来源于鸽子胸肌, ≥95% based on Mol. Wt. 12,173 basis

C4879

α-糜蛋白酶原A 来源于牛胰腺, essentially salt-free, lyophilized powder

C2037

线粒体蛋白 来源于牛心脏, ≥95% based on Mol. Wt. 12,327 basis

C3131

线粒体蛋白 来源于牛心脏, ≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture

C7150

细胞色素 C 来源于马心脏, suitable for GFC marker, BioReagent

C7752

细胞色素 C 来源于马心脏, ≥95% based on Mol. Wt. 12,384 basis

C2506

细胞色素 C 来源于马心脏, ≥95% (SDS-PAGE)

C2436

细胞色素 C 来源于酿酒酵母, ≥85% based on Mol. Wt. 12,588 basis

R5125

核糖核酸酶A 来源于牛胰腺, Type III-A, ≥85% RNase A basis (SDS-PAGE), 85-140 Kunitz units/mg protein

R5000

核糖核酸酶A 来源于牛胰腺, Type II-A, ≥60% (SDS-PAGE), >= 60 Kunitz units/mg protein

R6513

核糖核酸酶A 来源于牛胰腺, Molecular Biology, ≥70 Kunitz units/mg protein, lyophilized

R4642

核糖核酸酶A 来源于牛胰腺, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

R5500

核糖核酸酶A 来源于牛胰腺, Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein

R4875

核糖核酸酶A 来源于牛胰腺, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein

R5250

核糖核酸酶A 来源于牛胰腺, Type X-A, ≥90% (SDS-PAGE), ≥70 Kunitz units/mg protein

R5503

核糖核酸酶A 来源于牛胰腺, Type I-AS, 50-100 Kunitz units/mg protein

C4129

α-糜蛋白酶 来源于牛胰腺, Type II, lyophilized powder, ≥40 units/mg protein

C3483

线粒体蛋白 来源于人类心脏, ≥95% (SDS-PAGE), lyophilized powder

30398

细胞色素 C 来源于牛心脏, ≥95% (GE)

C2867

细胞色素 C 来源于马心脏, BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture

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