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C2506

Sigma-Aldrich

细胞色素 C 来源于马心脏

≥95% (SDS-PAGE)

别名:

Cytochrome c 来源于马心脏

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About This Item

CAS号:
9007-43-6
EC 号:
232-700-9
MDL编号:
MFCD00130890
UNSPSC代码:
12352202
NACRES:
NA.61

生物来源

horse heart

质量水平

300

检测方案

≥95% (SDS-PAGE)

形式

powder

分子量

12,384

技术

cell based assay: suitable

溶解性

H2O: soluble 10 mg/mL

适用性

suitable for molecular biology

UniProt登记号

P00004

应用

cell analysis

储存温度

−20°C

基因信息

horse ... CYCS(100053958)

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应用

细胞色素c已用于：
  • 还原由黄嘌呤氧化酶生成的超氧自由基-铁氧化铬c
  • 采用MALDI质谱法，用单液滴沉积法进行分析
  • 作为标准细胞色素c还原酶活性测定的末端电子受体
  • 细胞色素c氧化酶组织化学
  • 用于细胞色素C氧化酶活性的测定
  • 作为磷酸盐缓冲液的组分，用于采用蓝色天然凝胶电泳 (BN-PAGE) 检测和评估复合物 IV 活性
  • 用于检测离体肾脏吞噬细胞中的细胞外超氧阴离子 (ECSA)
马的细胞色素c经过H2O2和UV 处理后的特异性位点和氧化程度已经过表征。研究表明这些位点可能可用作细胞内氧化环境的生物标记。

生化/生理作用

细胞色素 c 从线粒体外层释放到胞质溶胶中，有助于激活 caspase-9 并启动凋亡蛋白酶级联反应。其与凋亡蛋白酶激活因子-1 (APAF-1) 和三磷酸脱氧腺苷 (dATP) 的相互作用可促进这一过程。(3)各种研究表明，测定肿瘤患者血清中的细胞色素 C 水平可以被认为是体内细胞死亡的潜在标志。
细胞色素c的主要身份是电子载体线粒体蛋白。在细胞内，细胞色素c能够在二价铁和三价铁之间转化，使得它称为了有效的生物电子载体，在动植物细胞内氧化中起到很重要的作用。它通常被视作呼吸作用催化剂，在呼吸底物和氧气之间形成必需的电子桥

制备说明

Prepared using TCA.

其他说明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)
想要了解更多细胞色素c和电子传递的信息，请访问www.sigma-aldrich.com/enzymeexplorer.

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. I see that you list several horse heart cytochrome c products. What are the differences, and which one should I choose?

    Product No. C2506 is prepared with trichloroacetic acid (TCA), whereas C7752 is prepared with the milder acetic acid. The TCA method may reduce the amount of superoxide dismutase (SOD) present, but tends to cause dimerization or acid-modified structures of cytochrome c. In contrast, acetic acid preparations may have slightly higher amounts of SOD, but a lower proportion of dimeric cytochrome c. So C2506 would be recommended for enzyme activity studies that require low SOD, whereas C7752 is recommended for structural studies or binding studies, or for enzyme activity studies where possible SOD contamination is not a problem. Product No. C2867 is prepared from C2506; it is a BioUtra grade product, greater than or equal to 99% pure as determined by SDS-PAGE. It is recommended when an ultra-high purity is required.  Product No. C3256 is also prepared from C7752; it is isolated by ion-exchange chromatography. This fraction shows a high rate of ascorbic acid oxidation, but low activity in a cytochrome oxidase system.  Product No. C7150 is also prepared from C7752; it is suitable for use as a gel filtration molecular weight marker.

  6. How do I determine how much of Product C2506, Cytochrome c, is in the oxidized form, and how much is reduced?

    This is determined by a spectral assay. The detailed assay procedure (operating procedure #11252) may be requested from Sigma St. Louis Technical Service (techserv@sial.com).

  7. Is Product C2506, Cytochrome c, predominantly in the oxidized form or the reduced form?

    This product is predominantly (>95%) in the oxidized form.

  8. How should I store solutions of Product C2506, Cytochrome c?

    Our general recommendations are as follows:  Store at -20 °C (freezer) for up to six months. Store at 2-8 °C (refrigerator) for up to two weeks. Store at 20-25 °C (room temperature) for up to three days. According to J. Biol. Chem, 237, 2161-2174 (1962), cytochrome c is stable to boiling, and over an unusually wide range of pH values. However, it denatures readily at extreme pH (low or high), and in high concentrations of organic solvents, such as ethanol or acetone.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

B C Hill
The Journal of biological chemistry, 266(4), 2219-2226 (1991-02-05)
The reaction of the electrostatic cytochrome c-cytochrome oxidase complex with oxygen is measured by transient absorption spectroscopy. The oxygen reaction is initiated by photolytic removal of CO from cytochrome oxidase, using a flash-pumped dye laser. The subsequent reaction of the
Enzyme-catalyzed free radical reactions with nicotinamide adenine nucleotides. II. Lactate dehydrogenase-catalyzed oxidation of reduced nicotinamide adenine dinucleotide by superoxide radicals generated by xanthine oxidase.
P C Chan et al.
The Journal of biological chemistry, 249(4), 1317-1319 (1974-02-25)
A novel role of the mitochondrial permeability transition pore in (-)-gossypol-induced mitochondrial dysfunction
Warnsmann V, et al.
Mechanisms of Ageing and Development, 170, 45-58 (2018)
Vittorio Pandini et al.
The Journal of biological chemistry, 277(50), 48463-48471 (2002-10-09)
Toxoplasma gondii possesses an apicoplast-localized, plant-type ferredoxin-NADP(+) reductase. We have cloned a [2Fe-2S] ferredoxin from the same parasite to investigate the interplay of the two redox proteins. A detailed characterization of the two purified recombinant proteins, particularly as to their
?-1,3 glucan derived from Euglena gracilis and Algamune? enhances innate immune responses of red drum (Sciaenops ocellatus L.)
Yamamoto FY, et al.
Fish & Shellfish Immunology, 77, 273-279 (2018)
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