推荐产品
生物来源
bovine heart
质量水平
方案
≥95% based on Mol. Wt. 12,327 basis
表单
powder
分子量
12327 Da
储存条件
(Tightly closed Dry)
技术
cell culture | mammalian: suitable
杂质
0.40-0.50% Iron (anhydrous)
溶解性
water: 10 mg/mL, dark red-brown
UniProt登记号
储存温度
−20°C
基因信息
cow ... CYC1(512500)
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一般描述
研究领域：细胞凋亡
细胞色素C(Cyt C)是重要的线粒体蛋白质，位于线粒体膜内叶。在细胞核中编码为apo-细胞色素C。细胞色素C结合在膜上，或作为能量传递膜中的可溶性金属蛋白，存在于细菌、古菌和质体中。属于IIa类细胞色素，含有五配位血红素中心。
细胞色素C(Cyt C)是重要的线粒体蛋白质，位于线粒体膜内叶。在细胞核中编码为apo-细胞色素C。细胞色素C结合在膜上，或作为能量传递膜中的可溶性金属蛋白，存在于细菌、古菌和质体中。属于IIa类细胞色素，含有五配位血红素中心。
应用
细胞色素C（来自牛心）已用于：
- 作为检测溶液组分，检测人骨肉瘤和人肝癌细胞线粒体中呼吸链复合体III和IV的活性
- 测定人主动脉内皮细胞线粒体中细胞色素C氧化酶的活性
- 作为细胞色素C氧化酶反应液组分，用于小鼠肾组织中细胞色素C氧化酶的原位染色
生化/生理作用
在细胞内，细胞色素c可在亚铁态和三价铁态之间迅速转化，这使其成为有效的生物电子转运体。它在动植物的细胞氧化过程中发挥重要作用。它通常被视作呼吸作用的通用催化剂，可在呼吸底物与氧气之间形成必要的电子桥。
细胞色素C(Cyt C)在caspase依赖性内源性凋亡途径参与激活caspase，通过凋亡启动细胞程序性死亡。具有脂结合活性，与心磷脂的相互作用与超氧化酶活性有关。细胞色素C在细胞色素C血红素裂解酶的帮助下结合血红素 ，释放到线粒体膜间隙中。
细胞色素c可在亚铁态和三价铁态之间迅速转化，这使其成为有效的生物电子载体。它在动植物的细胞氧化过程中发挥重要作用。通常视作是呼吸链中的通用连接，它在可呼吸底物与氧气之间形成必要的电子桥。
制备说明
醋酸制备，不需要TCA。
其他说明
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Stéphane T Gabilly et al.
Frontiers in plant science, 8, 1313-1313 (2017-08-12)
Cytochromes c are hemoproteins, with the prosthetic group covalently linked to the apoprotein, which function as electron carriers. A class of cytochromes c is defined by a CXXCH heme-binding motif where the cysteines form thioether bonds with the vinyl groups
C Garrido et al.
Cell death and differentiation, 13(9), 1423-1433 (2006-05-06)
In healthy cells, cytochrome c (Cyt c) is located in the mitochondrial intermembrane/intercristae spaces, where it functions as an electron shuttle in the respiratory chain and interacts with cardiolipin (CL). Several proapoptotic stimuli induce the permeabilization of the outer membrane
Increasing cGMP-dependent protein kinase I activity attenuates cisplatin-induced kidney injury through protection of mitochondria function
Hasiyeti M, et al.
American Journal of Physiology: Renal Physiology (2013)
Yong-Ling P Ow et al.
Nature reviews. Molecular cell biology, 9(7), 532-542 (2008-06-24)
Cytochrome c is primarily known for its function in the mitochondria as a key participant in the life-supporting function of ATP synthesis. However, when a cell receives an apoptotic stimulus, cytochrome c is released into the cytosol and triggers programmed
Michael A Hough et al.
Advances in microbial physiology, 67, 1-84 (2015-12-01)
Cytochromes c' are a group of class IIa cytochromes with pentacoordinate haem centres and are found in photosynthetic, denitrifying and methanotrophic bacteria. Their function remains unclear, although roles in nitric oxide (NO) trafficking during denitrification or in cellular defence against
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实验方案
Enzymatic Assay of Ferredoxin NADP Reductase - To standardize a procedure for the enzymatic assay of Ferredoxin NADP Reductase (EC.1.18.12).
Chromatograms
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