C4879
α-糜蛋白酶原A 来源于牛胰腺
essentially salt-free, lyophilized powder
别名:
chymotrypsin A zymogen
purified by
6× crystallization
biological source
bovine pancreas
type
Type II
form
essentially salt-free, lyophilized powder
specific activity
≥40 units/mg solid
mol wt
25,656 Da by calculation
solubility
1 mM HCl: soluble 10 mg/mL, clear, colorless
UniProt accession no.
foreign activity
α-chymotrypsin ≤1 U/mg (prior to activation by trypsin)
storage temp.
−20°C
Quality Level
Gene Information
cow ... CTRB1(618826)
General description
来自牛胰腺的胰凝乳蛋白酶原是一种含有5个二硫键的酶原。它的等电点是8.97。
Application
α-来自牛胰腺的胰凝乳蛋白酶原A已作为蛋白质模型用于结晶重现性研究。它还被用于质谱研究前α-麦醇溶蛋白的水解。
Sigma公司的这种酶已用于使用近红外（NIR）光谱技术无创测定固态蛋白质构象的过程中。它被用作研究蛋白质在聚合物/聚合物双水相体系中的分配。这种酶也被用作自相互作用色谱应用，以测试蛋白质渗透第二维利系数的快速测量。在该技术中，将蛋白质固定在色谱微粒上，并使用等度替代测定其保留时间。
Biochem/physiol Actions
一种丝氨酸蛋白酶，水解肽键与肽键羧基末端的芳香族或大型疏水侧链 (Tyr、Trp、Phe、Met) 的肽键。
胰凝乳蛋白酶原A需要有限的蛋白水解才能激活。胰凝乳蛋白酶原A可由胰蛋白酶和胰凝乳蛋白酶激活（自溶激活）以形成 m α、β、γ、δ和π胰凝乳蛋白酶（取决于激活条件）。胰凝乳蛋白酶原是一种蛋白酶，优先裂解芳香族氨基酸（包括色氨酸、酪氨酸和苯丙氨酸）羧基侧的肽。它也水解亮氨酸、蛋氨酸和丙氨酸羧基侧的肽。
Other Notes
有关胰凝乳蛋白酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer
活化为硝酸凝乳蛋白酶后，在 pH 7.8、25°C 条件下，1 个单位将每分钟水解 1.0 μmol BTEE。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
低风险生物材料
此项目有
Simulation of the activation of alpha-chymotrypsin: Analysis of the pathway and role of the propeptide
Matrai J, et al.
Protein Science, 13(12), 3139-3150 (2004)
Rob Haselberg et al.
Analytica chimica acta, 678(1), 128-134 (2010-09-28)
A capillary electrophoresis-mass spectrometry (CE-MS) method using sheath liquid electrospray ionization interfacing was studied and optimized for the analysis of intact basic proteins. To prevent protein adsorption, capillaries with a noncovalent positively charged coating were utilized. Capillaries were coated by
Angelina Angelova et al.
International journal of pharmaceutics, 454(2), 625-632 (2013-06-25)
Defining appropriate delivery strategies of therapeutic proteins, based on lipid nanoparticulate carriers, requires knowledge of the nanoscale organization that determines the loading and release properties of the nanostructured particles. Nanoencapsulation of three cationic proteins (human brain-derived neurotrophic factor (BDNF), α-chymotrypsinogen
Structural basis of IgE binding to alpha-and gamma-gliadins: Contribution of disulfide bonds and repetitive and nonrepetitive domains
Mameri H, et al.
Journal of Agricultural and Food Chemistry, 63(29), 6546-6554 (2015)
9.1 Proteases: Facilitating a Difficult Reaction
Biochemistry (5th Edition) (2002)
商品
Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
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