等级
for molecular biology
质量水平
形式
lyophilized
比活
≥70 Kunitz units/mg protein
分子量
13.7 kDa
~13,700
异质活性
Exonuclease and endonuclease, none detected
NICKase and DNase, none detected
储存温度
−20°C
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
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相关类别
一般描述
应用
- RNase A用于去除DNA质粒和基因组DNA制品和蛋白质样品中的RNA。
- RNase A还用于RNA序列分析和保护测定。
- RNase A已用作计算辅助药物设计的工具。
- RNase A为RNA序列分析提供支持。
- RNase A水解蛋白质样品中的RNA。
- RNase A为DNA纯化提供支持。
- 质粒DNA纯化
- 在细胞核染色过程中，酶解双链RNA并避免染色
- 免疫过氧化物酶和免疫荧光检测
- 流式细胞分析前的细胞处理
- 用作杂交后缓冲液组分，在原位杂交后洗涤FFPE组织切片
- 在制备外膜蛋白(OMP)提取物期间处理细胞
- 核糖核酸酶保护试验
- 去除非特异性结合的RNA
- 核糖核酸序列的分析
- 水解蛋白质样品所含的RNA
特点和优势
分析说明
其他说明
该RNase A产品不需要煮沸原液以灭活剩余的DNase，这有可能导致RNase沉淀，并可能导致酶活性损失。如果RNase溶液在中性pH下加热，就会发生沉淀。当在较低的pH值下加热时，由于存在蛋白质杂质，可能会发生一些沉淀。
应用
抑制剂
相关产品
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
What is the solubility of Product R6513, Ribonuclease A from bovine pancreas (RNase A)?
Stock solutions of RNase A can be made in 10 mM Tris-HCl, pH 7.5, 15 mM NaCl at a concentration of 10 mg/mL.
What is the shelf life of Product R6513, Ribonuclease A from bovine pancreas (RNase A)?
This product is stable for at least 3 years when stored properly at -20 °C.
How are solutions of Product R6513, Ribonuclease A from bovine pancreas (RNase A), stored?
RNase A solutions can be stored, aliquoted and frozen at -20 °C.
Do I need to boil Product R6513, Ribonuclease A from bovine pancreas (RNase A), to inactivate residual DNases?
Boiling of stock solutions to inactivate residual DNase is not necessary and may cause precipitation of RNase with loss of enzymatic activity.
What working concentration of Product R6513, Ribonuclease A from bovine pancreas (RNase A), do you suggest?
We suggest using RNase A at a concentration of 10 ug/ml.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Why is RNase A activity not listed as micromoles per minute, and what is a Kunitz unit?
The Kunitz unit was created to reflect the non-linear nature of the RNAse enzymatic reaction. As the reaction progresses the activity changes due to RNA as a heterogeneous polymeric substrate; therefore, the unit of moles or micomoles per minute becomes inaccurate. As such, our enzymatic assay describes a unit that varies throughout the reaction: One unit will cause a decrease of 100% per minute in the value of E0 - Ef at pH 5.0 at 25°C. Ef is determined in the RNAse control step, while E0 reflects the current reaction rate.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
实验方案
本实验方案可用于测定核糖核酸酶A(RNase A)的活性。
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
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