等级
for molecular biology
形式
buffered aqueous glycerol solution
分子量
~80 kDa
浓度
≥10,000 units/mL
UniProt登记号
异质活性
DNase, RNase, none detected
运输
wet ice
储存温度
−20°C
基因信息
cow ... ALPI(280993)
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一般描述
碱性磷酸酶是一种磷酸单酯酶，可催化核酸的末端磷酸盐裂解。游离 5′-OH 可用多核苷酸激酶和 γ32P-ATP 磷酸化，以产生 γ32P 末端标记核酸。通过碱性磷酸酶去磷酸化可以防止线性化的克隆载体再环化。
应用
通常用于在分子克隆反应过程中从核酸中去除5'-末端磷酸盐。
组分
碱性磷酸酶以50% (w/v) 甘油溶液的形式提供，溶液中还含有 10 mM Tris-HCl (pH 8.2)、50 mM KCl、1 mM MgCl 2 和 0.1 mM ZnCl 2 。还包括 10X CIP 缓冲液（产品编号 C3225）。
单位定义
一个单位将在37℃ 下每分钟水解 1.0μ摩尔对 -硝基苯磷酸盐。
其他说明
5 mM EDTA (pH 8) 中的碱性磷酸酶将在 75℃ 下不可逆地加热灭活 10 分。
抑制剂
相关产品
产品编号
说明
价格
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
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Scaffold architecture, surface topography, biochemical and mechanical cues have been shown to significantly improve cellular events and in vivo tissue regeneration. Specifically electrospun nanofiber matrices have gained tremendous interest due to their intrinsic structural resemblance to native tissue extracellular matrix
实验方案
CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.
CIP可用于脱去DNA、RNA及核糖和脱氧核糖核苷三磷酸上的5’-磷酸基团。如何进行DNA去磷酸化的详细实验方案。
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
碱性磷酸酶的酶法测定，二乙醇胺法（EC 3.1.3.1)
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