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主要文件

安全信息

P4978

Sigma-Aldrich

磷酸酶(碱性) 来源于小牛肠

buffered aqueous glycerol solution

别名:

CIAP, CIP, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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1 G
¥1,808.60
5 G
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1 G
¥1,808.60
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About This Item

CAS号:
MDL编号:
UNSPSC代码:
12352204
eCl@ss:
42010105
NACRES:
NA.53

¥1,808.60


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等级

Molecular Biology

表单

buffered aqueous glycerol solution

分子量

~80 kDa

浓度

≥10,000 units/mL

UniProt登记号

异质活性

DNase, RNase, none detected

运输

wet ice

储存温度

−20°C

基因信息

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此商品
H9039MBD00523741
biological source

Porcine

biological source

bovine

biological source

-

biological source

-

assay

≥96.0% (HPLC)

assay

≥90%

assay

-

assay

-

technique(s)

tissue culture: suitable, transfection: suitable

technique(s)

toxicology assay: suitable

technique(s)

-

technique(s)

-

form

powder or crystals

form

powder

form

liquid

form

solid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

15-25°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

一般描述

碱性磷酸酶是一种磷酸单酯酶,可催化核酸的末端磷酸盐裂解。游离 5′-OH 可用多核苷酸激酶和 γ32P-ATP 磷酸化,以产生 γ32P 末端标记核酸。通过碱性磷酸酶去磷酸化可以防止线性化的克隆载体再环化。

应用

通常用于在分子克隆反应过程中从核酸中去除5'-末端磷酸盐。

组分

碱性磷酸酶以50% (w/v) 甘油溶液的形式提供,溶液中还含有 10 mM Tris-HCl (pH 8.2)、50 mM KCl、1 mM MgCl 2 和 0.1 mM ZnCl 2 。还包括 10X CIP 缓冲液(产品编号 C3225)。

单位定义

一个单位将在37℃ 下每分钟水解 1.0μ摩尔对 -硝基苯磷酸盐。

其他说明

5 mM EDTA (pH 8) 中的碱性磷酸酶将在 75℃ 下不可逆地加热灭活 10 分。

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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    Lars Hansen et al.
    American journal of human genetics, 92(4), 575-583 (2013-04-09)
    PGAP2 encodes a protein involved in remodeling the glycosylphosphatidylinositol (GPI) anchor in the Golgi apparatus. After synthesis in the endoplasmic reticulum (ER), GPI anchors are transferred to the proteins and are remodeled while transported through the Golgi to the cell

    实验方案

    CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.

    CIP可用于脱去DNA、RNA及核糖和脱氧核糖核苷三磷酸上的5’-磷酸基团。如何进行DNA去磷酸化的详细实验方案。

    Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

    碱性磷酸酶的酶法测定,二乙醇胺法(EC 3.1.3.1)

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