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Key Documents

Safety Information

D4889

Sigma-Aldrich

Deoxyribonucleic acid sodium salt from Escherichia coli strain B

Genomic, unsheared

Synonym(s):

DNA

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20 EA
¥3,850.41

¥3,850.41

Available to ship on2025年4月27日Details

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20 EA
¥3,850.41

About This Item

CAS Number:
93384-16-8
EC Number:
297-226-7
MDL number:
MFCD00130922
UNSPSC Code:
41106310
eCl@ss:
32160414
NACRES:
NA.52

¥3,850.41

Available to ship on2025年4月27日Details

grade

Molecular Biology

Quality Level

200

form

lyophilized powder

mol wt

>45 kb

storage temp.

2-8°C

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Show Differences

1 of 4

This Item
853086853001852164
Boc-Trp-OH Novabiochem®

853038

Boc-Trp-OH

Boc-D-Trp-OH Novabiochem®

853086

Boc-D-Trp-OH

Boc-Ala-OH Novabiochem®

853001

Boc-Ala-OH

Fmoc-D-Trp(Boc)-OH Novabiochem®

852164

Fmoc-D-Trp(Boc)-OH

form

powder

form

powder

form

powder

form

powder

assay

≥98% (TLC)

assay

≥98% (TLC)

assay

≥98% (TLC)

assay

≥85.0% (acidimetric), ≥98% (TLC), ≥98.0% (HPLC)

product line

Novabiochem®

product line

Novabiochem®

product line

Novabiochem®

product line

Novabiochem®

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

15-25°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

reaction suitability

reaction type: Boc solid-phase peptide synthesis

reaction suitability

reaction type: Boc solid-phase peptide synthesis

reaction suitability

reaction type: Boc solid-phase peptide synthesis

reaction suitability

reaction type: Fmoc solid-phase peptide synthesis

General description

Escherichia coli strain B is most widely studied DNA after k-12. The genomes of E coli strain B and k-12 show differences in the IS sequence elements. Strain B have B-islands and express O-specific side-chain LPS.[1] The product is unsheared genomic DNA.

Specificity

50% GC; Tm = 90.5 °C

Application

The DNAs are particularly useful for genomic analysis, including PCR, library construction in bacteriophage λ, and in heterologous pre-hybridization and hybridization protocols.
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used for:
  • PCR reactions of genomic DNA of Gardnerella vaginalis[2]
  • nick translation and radiolabeling of dNTP[3]
  • generating standard curve for 16S rDNA quantification[4]
  • transfecting neutrophils[5]
  • testing the extraction methods[6]

Unit Definition

One unit will yield an A260 of 1.0 in 1.0 ml of 1 mM Tris-HCl, pH 7.5, with 1 mM NaCl and 1 mM EDTA (1 cm light path).

Preparation Note

Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of approx. 2 units per mL. Approx. 20 A260 units per mg DNA. % GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.

Analysis Note

Analyzed by gel electrophoresis and visualization after ethidium bromide staining, with single predominant band of 45 kB or greater.

Other Notes

DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000277851
    0000365132
    0000365132
    0000277851
    SLCR5501

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    Find documentation for the products that you have recently purchased in the Document Library.

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    Yue Huo et al.
    Current microbiology, 76(1), 22-28 (2018-11-02)
    A Gram-positive bacterium (DCY118T) was isolated from ginseng-cultivated soil in Gochang-gun, Republic of Korea. This isolate was assigned to the genus Ornithinimicrobium and is closely related to Ornithinimicrobium kibberense K22-20T (98.8%), O. pekingense DSM 21552T (98.5%), O. algicola JC311T (98.2%)
    Yue Huo et al.
    Archives of microbiology, 202(6), 1341-1347 (2020-03-11)
    A new bacterium, designated DCY113T, was isolated from ginseng cultivation soil in Gochang-gun, South Korea, and its taxonomic position identified by the polyphasic approach. 16S rRNA gene sequence analysis determined that this isolate belongs to the genus Paraburkholderia, and was
    Benito J Regueiro et al.
    PloS one, 5(10), e13387-e13387 (2010-10-23)
    Sepsis is one of the leading causes of morbidity and mortality in hospitalized patients worldwide. Molecular technologies for rapid detection of microorganisms in patients with sepsis have only recently become available. LightCycler SeptiFast test M(grade) (Roche Diagnostics GmbH) is a
    J H Paul et al.
    Applied and environmental microbiology, 53(1), 170-179 (1987-01-01)
    The production and turnover of dissolved DNA in subtropical estuarine and oligotrophic oceanic environments were investigated. Actively growing heterotrophic bacterioplankton (i.e., those capable of [3H]thymidine incorporation) were found to produce dissolved DNA, presumably through the processes of death and lysis
    M Agarwal et al.
    BJU international, 88(9), 868-870 (2002-02-20)
    To investigate the possible role of Gardnerella vaginalis in interstitial cystitis (IC), using molecular methods to avoid difficulties with the culture and recovery of viable organisms, and the problems associated with the recovery of low numbers of culturable organisms. Thirty-three
    View All Related Papers

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