质量水平
100
200
形式
liquid (2x concentrate)
异质活性
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
储存温度
−20°C
一般描述
Sigma的预杂交液是一种2倍浓缩的即用型杂交缓冲液，适用于Northern和Southern印迹方案。
应用
适合用作Northern和Southern印迹方案的预杂交缓冲液。
组分
Sigma的2倍预杂交液含200 μg/mL DNA，溶剂为10倍SSC和2倍Denhardt溶液：
- 200 μg/mL DNA
- 2mg牛血清白蛋白（BSA）
- 2mg聚蔗糖
- 2mg聚乙烯吡咯烷酮（PVP）
- 0.15 M柠檬酸钠
- 1.5M NaCl
相关产品
产品编号
说明
价格
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Sambrook, J., et al.
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实验方案
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
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