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应用
大肠杆菌DNA在研究中具有多种用途。 大肠杆菌DNA（ecDNA）可用于研究能够调节DNA结构和功能的DNA结合剂。 大肠杆菌DNA可用于溶液中DNA行为的物理化学研究，并评估物种特异性DNA的结构和结合结构域。该DNA对于基因组分析，包括PCR、噬菌体λ中的文库构建以及异源预杂交和杂交实验方案特别有用。
来自大肠杆菌B菌株的脱氧核糖核酸钠盐已被用于测量解链温度（Tm）和差异扫描量热法（DSC）研究中。它也已被用作聚合酶链反应（PCR）中的模板序列。
制备说明
按照Marmur, J., J. Mol. Biol., 3, 208 (1961)中的过程制备而得。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Mitsumasa Hashimoto et al.
The Journal of biological chemistry, 278(31), 28501-28507 (2003-05-16)
Closely opposed lesions form a unique class of DNA damage that is generated by ionizing radiation. Improper repair of closely opposed lesions could lead to the formation of double strand breaks that can result in increased lethality and mutagenesis. In
Differential scanning calorimetry of bacteria
Miles CA, et al.
Microbiology, 132(4), 939-952 (1986)
R Richins et al.
Biotechnology progress, 17(2), 252-257 (2001-04-21)
The Escherichia coli DNA binding protein FIS is a transcriptional modulator involved in the regulation of many cellular processes, including the activation of rRNA synthesis. High-level overproduction of FIS in early, mid, or late log cultures resulted in growth-phase- and
O Schröder et al.
Biochemical and biophysical research communications, 282(1), 219-227 (2001-03-27)
The DNA-binding protein H-NS compacts DNA and acts as a specific transcription factor regulating the expression of various bacterial genes. The small abundant protein binds to curved DNA without apparent sequence specificity and the exact nature of its DNA interaction
Alasdair I MacDonald et al.
Plasmid, 59(2), 119-126 (2008-01-30)
In the lysogenic state, bacteriophage P1 is maintained as a low copy-number circular plasmid. Site-specific recombination at loxP by the phage-encoded Cre protein keeps P1 monomeric, thus helping to ensure stable plasmid inheritance. Two Escherichia coli DNA-binding proteins, PepA and
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