一般描述
适合在 Southern 杂交中用作封闭剂。
应用
在核酸杂交中，有许多因素影响信噪比。这些因素包括溶剂（甲酰胺）的存在、杂交温度、杂交长度、杂交溶液的体积、搅拌的程度和方法、封闭试剂的使用、探针的浓度和比活度、使用分子制剂以提高核酸再结合率以及洗膜过程中使用的严格程度。
为了减少探针与底物的非特异性杂交，必须使用封闭剂。一般结合采用封闭剂、去污剂和变性、片段化 DNA来实现此目的。Sigma 提供来自多种物种的超声变性 DNA，在RNA印迹和 DNA印迹以及其他核酸杂交技术中用作封闭剂。
为了减少探针与底物的非特异性杂交，必须使用封闭剂。一般结合采用封闭剂、去污剂和变性、片段化 DNA来实现此目的。Sigma 提供来自多种物种的超声变性 DNA，在RNA印迹和 DNA印迹以及其他核酸杂交技术中用作封闭剂。
来自鲱鱼精子的脱氧核糖核酸适合用作Southern杂交的封闭剂。可用于制备 BrdU 免疫组化小鼠脑切片。也用于斑点印迹膜的杂交反应。
制备说明
将该 DNA 进行酚-氯仿提取、乙醇沉淀和超声处理以产生与 587 和 831 碱基对标记片段相同的单链片段。
其他说明
溶液中的 DNA 将在室温下静置后重新退火，因此建议将溶液煮沸 10 分钟，然后在使用前在冰上冷却至少 5 分钟。
相关产品
产品编号
说明
价格
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
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