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安全信息

N6019

Sigma-Aldrich

Neutralizing Buffer

for Northern and Southern blotting, powder

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别名:
Neutralizing Buffer for Blotting Techniques
UNSPSC代码:
12352200
NACRES:
NA.25

等级

for molecular biology

质量水平

200

形式

powder

技术

Southern blotting: suitable

异质活性

DNase, RNase, none detected

储存温度

room temp

相关类别

一般描述

Sigma′s neutralization buffer is suitable for Southern blotting procedures to neutralize the pH of the gel following depurination and denaturation treatment or before transferring it to membranes.

应用

Suitable for neutralizing the gel before transfer to membranes during Southern procedures.

组分

The Neutralization Buffer is composed of 1M Tris-HCl and 1.5M NaCl.

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

监管及禁止进口产品

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访问文档库

A southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures
Cai D, et al.
Methods in Molecular Biology, 151-161 (2013)
Sambrook, J., and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 6-6 (2001)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Ausubel, F.M., et al.
Short Protocols in Molecular Biology (1995)

实验方案

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

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