等级
for molecular biology
质量水平
形式
powder
技术
Southern blotting: suitable
异质活性
DNase, RNase, none detected
储存温度
room temp
一般描述
Sigma′s neutralization buffer is suitable for Southern blotting procedures to neutralize the pH of the gel following depurination and denaturation treatment or before transferring it to membranes.
应用
Suitable for neutralizing the gel before transfer to membranes during Southern procedures.
组分
The Neutralization Buffer is composed of 1M Tris-HCl and 1.5M NaCl.
相关产品
产品编号
说明
价格
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
A southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures
Cai D, et al.
Methods in Molecular Biology, 151-161 (2013)
Ausubel, F.M., et al.
Short Protocols in Molecular Biology (1995)
Sambrook, J., and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 6-6 (2001)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
实验方案
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
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