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Merck
CN

15356

Sigma-Aldrich

Blotting-Nylon 66 membranes, type B, positive

for Northern and Southern blotting

别名:

Nylon 66-Blotting membranes, type B

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About This Item

UNSPSC代码:
41105339
NACRES:
NA.25

物料

Nylon

质量水平

等级

for molecular biology

形式

roll

表面覆盖度

surface (quaternary ammonium groups) ≥99%

孔径

0.45 μm pore size

储存温度

room temp

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一般描述

Positively charged nylon cast on polyester-support; membrane size 0,2m x 3m (rolled up)

应用

Suitable for:
Protein transfer
Radioactive detection of blotted proteins and nucleic acids
Sensitive dot and slot blots of nucleic acids
for high-sensitive nucleic acid blotting, dot and lot blots. Particularly suitable for radioactive detection. Suitable for protein transfer and immunoblotting.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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分析证书(COA)

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访问文档库

Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Gerardas Dambrauskas et al.
Journal of experimental botany, 54(383), 699-706 (2003-01-30)
Very little is known about the expression patterns in plants of genes that encode proteins involved in the initiation of DNA replication. Partial cDNA sequences that encode Cdc6 and Mcm3 in tobacco have been isolated. The sequences were used as
K Pfeifer et al.
Journal of cell science, 106 ( Pt 2), 545-553 (1993-10-01)
Ubiquitination of proteins is a critical step in the controlled degradation process of many polypeptides. Here we show that sponges, the simplest multicellular group of eukaryotic organisms, are also equipped with the ubiquitin pathway. The polyubiquitin cDNA was isolated and

实验方案

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

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