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Merck
CN

15356

Blotting-Nylon 66 membranes, type B, positive

for Northern and Southern blotting

别名:

Nylon 66-Blotting membranes, type B

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关于此项目

NACRES:
NA.25
UNSPSC Code:
41105339
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产品名称

Blotting-Nylon 66 membranes, type B, positive, for Northern and Southern blotting

material

Nylon

grade

Molecular Biology

form

roll

surface coverage

surface (quaternary ammonium groups) ≥99%

pore size

0.45 μm pore size

storage temp.

room temp

Quality Level

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Application

Suitable for:
Protein transfer
Radioactive detection of blotted proteins and nucleic acids
Sensitive dot and slot blots of nucleic acids
for high-sensitive nucleic acid blotting, dot and lot blots. Particularly suitable for radioactive detection. Suitable for protein transfer and immunoblotting.

General description

Blotting nylon provided on a roll is a standard item used in transfer of protein or nucleic acids during Western, Southern and Northern procedures. This membrane is positively charged nylon cast on a polyester support. It comes as a roll sized 0.2m x 3m, and can be cut to any size.

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Gerardas Dambrauskas et al.
Journal of experimental botany, 54(383), 699-706 (2003-01-30)
Very little is known about the expression patterns in plants of genes that encode proteins involved in the initiation of DNA replication. Partial cDNA sequences that encode Cdc6 and Mcm3 in tobacco have been isolated. The sequences were used as
K Pfeifer et al.
Journal of cell science, 106 ( Pt 2), 545-553 (1993-10-01)
Ubiquitination of proteins is a critical step in the controlled degradation process of many polypeptides. Here we show that sponges, the simplest multicellular group of eukaryotic organisms, are also equipped with the ubiquitin pathway. The polyubiquitin cDNA was isolated and
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)

实验方案

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

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