跳转至内容
Merck
CN
所有图片(1)

文件

S6639

Sigma-Aldrich

SSC 20x 浓缩缓冲液

for Northern and Southern blotting, solution

登录查看公司和协议定价
所有图片(1)
别名:
20X SSC Buffer, SSC hybridization buffer, Saline-sodium citrate buffer
UNSPSC代码:
41105319
NACRES:
NA.25

等级

for molecular biology

质量水平

200

描述

Saline-Sodium Citrate buffer, made with ultrapure water

无菌性

0.2 μm filtered

形式

solution

应用

diagnostic assay manufacturing

异质活性

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

储存温度

room temp

正在寻找类似产品? 访问 产品对比指南

相关类别

一般描述

SSC（柠檬酸钠盐）缓冲液20倍浓缩液是Southern和Northern杂交过程的标准试剂。 它可用于严格控制杂交后洗涤步骤所用洗涤缓冲液。

应用

SSC缓冲液20倍浓缩液已用于
  • 用作原位杂交人特异性重复Alu序列的杂交缓冲液
  • 用作Southern杂交、小鼠基因组DNA斑点印迹和microRNA的Northern印迹的印迹膜洗涤缓冲液
  • 用作L1反转录转座子荧光原位杂交(FISH)的孵育缓冲液

特点和优势

  • 方便的即用型液体
  • 在杂交中具有多种用途

组分

SSC缓冲液20倍浓缩液由3M氯化钠、0.3M柠檬酸钠组成（pH7.0）。

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Chronic hypoxia facilitates Alzheimer's disease through demethylation of gamma-secretase by downregulating DNA methyltransferase 3b
Liu H, et al.
Alzheimers Dement., 12(2), 130-143 (2016)
L1 retrotransposon expression in circulating tumor cells
Papasotiriou I, et al.
Testing, 12(2), e0171466-e0171466 (2017)
Chang-Gong Liu et al.
Methods (San Diego, Calif.), 44(1), 22-30 (2007-12-26)
After 12 years from its first application, microarray technology has become the reference technique to monitor gene expression of thousands of genes in the same experiment. In the past few years an increasing amount of evidence showed the importance of
M Böttger et al.
Nucleic acids research, 9(20), 5253-5268 (1981-10-24)
Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component
Michaela Asp et al.
Scientific reports, 7(1), 12941-12941 (2017-10-13)
Heart failure is a major health problem linked to poor quality of life and high mortality rates. Hence, novel biomarkers, such as fetal marker genes with low expression levels, could potentially differentiate disease states in order to improve therapy. In
查看所有相关文献

商品

荧光原位杂交（FISH）

可用的荧光原位杂交（FISH）过程、试剂和设备。

Fluorescent in situ Hybridization (FISH)

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

实验方案

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门