等级
for molecular biology
质量水平
描述
Saline-Sodium Citrate buffer, made with ultrapure water
无菌性
0.2 μm filtered
形式
solution
应用
diagnostic assay manufacturing
异质活性
DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected
储存温度
room temp
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一般描述
SSC（柠檬酸钠盐）缓冲液20倍浓缩液是Southern和Northern杂交过程的标准试剂。 它可用于严格控制杂交后洗涤步骤所用洗涤缓冲液。
应用
SSC缓冲液20倍浓缩液已用于
- 用作原位杂交人特异性重复Alu序列的杂交缓冲液
- 用作Southern杂交、小鼠基因组DNA斑点印迹和microRNA的Northern印迹的印迹膜洗涤缓冲液
- 用作L1反转录转座子荧光原位杂交(FISH)的孵育缓冲液
特点和优势
- 方便的即用型液体
- 在杂交中具有多种用途
组分
SSC缓冲液20倍浓缩液由3M氯化钠、0.3M柠檬酸钠组成（pH7.0）。
相关产品
产品编号
说明
价格
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Chang-Gong Liu et al.
Methods (San Diego, Calif.), 44(1), 22-30 (2007-12-26)
After 12 years from its first application, microarray technology has become the reference technique to monitor gene expression of thousands of genes in the same experiment. In the past few years an increasing amount of evidence showed the importance of
Michaela Asp et al.
Scientific reports, 7(1), 12941-12941 (2017-10-13)
Heart failure is a major health problem linked to poor quality of life and high mortality rates. Hence, novel biomarkers, such as fetal marker genes with low expression levels, could potentially differentiate disease states in order to improve therapy. In
M Böttger et al.
Nucleic acids research, 9(20), 5253-5268 (1981-10-24)
Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component
Chronic hypoxia facilitates Alzheimer's disease through demethylation of gamma-secretase by downregulating DNA methyltransferase 3b
Liu H, et al.
Alzheimers Dement., 12(2), 130-143 (2016)
L1 retrotransposon expression in circulating tumor cells
Papasotiriou I, et al.
Testing, 12(2), e0171466-e0171466 (2017)
商品
可用的荧光原位杂交（FISH）过程、试剂和设备。
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
实验方案
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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