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安全信息

D9156

Sigma-Aldrich

脱氧核糖核酸，单链 来源于鲑鱼睾丸

For hybridization

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别名:
single-stranded template DNA
CAS号:
68938-01-2
MDL编号:
MFCD00130921
UNSPSC代码:
41106310
eCl@ss:
32160414
NACRES:
NA.52

生物来源

fish testis (salmon)

质量水平

200

等级

for molecular biology

检测方案

9-11 mg/mL (DNA concentration)

形式

solution

浓度

10 mg/mL in H2O

运输

dry ice

储存温度

−20°C

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相关类别

一般描述

是从鲑鱼睾丸分离优质单链模板DNA的即用型解决方案。 溶液的供应浓度为9-12 mg/ml。

应用

许多因素有助于提高核酸杂交的信噪比。 这些因素包括溶剂（甲酰胺）的存在、杂交温度、杂交长度、杂交溶液体积、搅拌程度和方法、封闭剂的使用、探针浓度和比活性、使用分子试剂提高核酸重缔合的速率以及在洗涤膜的严格程度。

为了减少探针与基质的任何非特异性杂交，必须使用封闭剂。 通常，使用封闭试剂、去污剂以及变性片段化DNA的组合来实现此目的。 Sigma提供来自多种物种的超声处理的变性DNA，可用作Northern和Southern印迹以及其他核酸杂交技术的封闭剂。
鲑鱼睾丸的单链脱氧核糖核酸适合用作Southern杂交中的封闭剂。用于骨髓活检样本 和人血单核细胞亚群的原位杂交组织化学研究。

制备说明

将该DNA用乙醇沉淀并超声处理，产生单链片段，其与587和831碱基对标记片段共迁移。

其他说明

DNA溶液要在室温下放置进行再退火，因此建议使用前将溶液煮沸10分钟，然后在冰上至少冷却5分钟。

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WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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H W Ziegler-Heitbrock et al.
Blood, 79(2), 503-511 (1992-01-15)
Cytokine expression was analyzed in CD14++ regular monocytes and in the novel subset of CD14+/CD16+ small monocytes. Biologic activity for tumor necrosis factor (TNF), interleukin-1 (IL-1), and IL-6 in the supernatant of elutriator-enriched, cell sorter-purified small monocytes was about 10-fold
Marion Herbette et al.
PLoS genetics, 17(7), e1009662-e1009662 (2021-07-07)
Segregation Distorter (SD) is a male meiotic drive system in Drosophila melanogaster. Males heterozygous for a selfish SD chromosome rarely transmit the homologous SD+ chromosome. It is well established that distortion results from an interaction between Sd, the primary distorting
J W Said et al.
Blood, 90(11), 4278-4282 (1997-12-31)
We have recently demonstrated the presence of Kaposi's sarcoma-associated herpesvirus (KSHV) in cultured bone marrow (BM) stromal dendritic cells from all patients with myeloma studied. To show that these findings were not an artifact of tissue culture, we performed in
N Milne et al.
FEMS yeast research, 20(2) (2019-09-27)
We present a teaching protocol suitable for demonstrating the use of EasyClone and CRISPR/Cas9 for metabolic engineering of industrially relevant yeasts Saccharomyces cerevisiae and Yarrowia lipolytica, using β-carotene production as a case study. The protocol details all steps required to
Zachary D Smith et al.
Nature, 511(7511), 611-615 (2014-08-01)
In mammals, cytosine methylation is predominantly restricted to CpG dinucleotides and stably distributed across the genome, with local, cell-type-specific regulation directed by DNA binding factors. This comparatively static landscape is in marked contrast with the events of fertilization, during which
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