跳转至内容
Merck
CN
所有图片(1)

文件

安全信息

D9156

Sigma-Aldrich

脱氧核糖核酸，单链 来源于鲑鱼睾丸

For hybridization

别名:

single-stranded template DNA

登录查看公司和协议定价
所有图片(1)

About This Item

CAS号:
68938-01-2
MDL编号:
MFCD00130921
UNSPSC代码:
41106310
eCl@ss:
32160414
NACRES:
NA.52

生物来源

fish testis (salmon)

质量水平

200

等级

for molecular biology

检测方案

9-11 mg/mL (DNA concentration)

形式

solution

浓度

10 mg/mL in H2O

运输

dry ice

储存温度

−20°C

正在寻找类似产品? 访问 产品对比指南

相关类别

一般描述

是从鲑鱼睾丸分离优质单链模板DNA的即用型解决方案。 溶液的供应浓度为9-12 mg/ml。

应用

许多因素有助于提高核酸杂交的信噪比。 这些因素包括溶剂（甲酰胺）的存在、杂交温度、杂交长度、杂交溶液体积、搅拌程度和方法、封闭剂的使用、探针浓度和比活性、使用分子试剂提高核酸重缔合的速率以及在洗涤膜的严格程度。

为了减少探针与基质的任何非特异性杂交，必须使用封闭剂。 通常，使用封闭试剂、去污剂以及变性片段化DNA的组合来实现此目的。 Sigma提供来自多种物种的超声处理的变性DNA，可用作Northern和Southern印迹以及其他核酸杂交技术的封闭剂。
鲑鱼睾丸的单链脱氧核糖核酸适合用作Southern杂交中的封闭剂。用于骨髓活检样本 和人血单核细胞亚群的原位杂交组织化学研究。

制备说明

将该DNA用乙醇沉淀并超声处理，产生单链片段，其与587和831碱基对标记片段共迁移。

其他说明

DNA溶液要在室温下放置进行再退火，因此建议使用前将溶液煮沸10分钟，然后在冰上至少冷却5分钟。

相关产品

产品编号
说明
价格

D4522

脱氧核糖核酸 来源于小牛胸腺, Type XV, Activated, lyophilized powder

D3287

Deoxyribonucleic acid, single stranded from human placenta

D4889

脱氧核糖核酸 钠盐 来源于大肠杆菌 菌株 B, Genomic, unsheared

D7290

脱氧核糖核酸 来源于鲱鱼精子, single-stranded for hybridization

D7656

脱氧核糖核酸，单链 来源于鲑鱼睾丸, For hybridization

D3035

脱氧核糖核酸 来源于人类胎盘, buffered aqueous solution, sexed, female

D3160

脱氧核糖核酸 来源于人类胎盘, buffered aqueous solution, sexed, male

D8661

脱氧核糖核酸 溶液 来源于小牛胸腺, For hybridization

S8140

SSPE Buffer, for Northern and Southern blotting, powder blend

N6019

Neutralizing Buffer, for Northern and Southern blotting, powder

N1531

Denaturation Solution, for Northern and Southern blotting

B6429

酪蛋白封闭缓冲液 10x, for Northern and Southern blotting, solution

S2015

SSPE 缓冲液 20× 浓缩液, for Northern and Southern blotting, solution

M5755

MOPS-EDTA-乙酸钠 (MESA), powder for RNA electrophoresis buffers

S0902

SSC缓冲剂, for Northern and Southern blotting, powder blend

H7033

PerfectHyb™ Plus杂交缓冲液, for Northern and Southern blotting, solution

S8015

SSC缓冲剂, for Northern and Southern blotting, powder blend

S6639

SSC 20x 浓缩缓冲液, for Northern and Southern blotting, solution

P1415

预杂交溶液, for Northern and Southern blotting, liquid (2x concentrate)

C7594

酪蛋白封闭缓冲液, for Northern and Southern blotting, powder blend

15356

Blotting-Nylon 66 membranes, type B, positive, for Northern and Southern blotting

31149

脱氧核糖核酸，低分子量 来源于鲑鱼精子

GE27-4565-01

Salmon Testes Sonicated DNA, Cytiva 27-4565-01, pack of 1 ea

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

H W Ziegler-Heitbrock et al.
Blood, 79(2), 503-511 (1992-01-15)
Cytokine expression was analyzed in CD14++ regular monocytes and in the novel subset of CD14+/CD16+ small monocytes. Biologic activity for tumor necrosis factor (TNF), interleukin-1 (IL-1), and IL-6 in the supernatant of elutriator-enriched, cell sorter-purified small monocytes was about 10-fold
J W Said et al.
Blood, 90(11), 4278-4282 (1997-12-31)
We have recently demonstrated the presence of Kaposi's sarcoma-associated herpesvirus (KSHV) in cultured bone marrow (BM) stromal dendritic cells from all patients with myeloma studied. To show that these findings were not an artifact of tissue culture, we performed in
Marion Herbette et al.
PLoS genetics, 17(7), e1009662-e1009662 (2021-07-07)
Segregation Distorter (SD) is a male meiotic drive system in Drosophila melanogaster. Males heterozygous for a selfish SD chromosome rarely transmit the homologous SD+ chromosome. It is well established that distortion results from an interaction between Sd, the primary distorting
N Milne et al.
FEMS yeast research, 20(2) (2019-09-27)
We present a teaching protocol suitable for demonstrating the use of EasyClone and CRISPR/Cas9 for metabolic engineering of industrially relevant yeasts Saccharomyces cerevisiae and Yarrowia lipolytica, using β-carotene production as a case study. The protocol details all steps required to
Manolis Kellis et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(17), 6131-6138 (2014-04-23)
With the completion of the human genome sequence, attention turned to identifying and annotating its functional DNA elements. As a complement to genetic and comparative genomics approaches, the Encyclopedia of DNA Elements Project was launched to contribute maps of RNA
查看所有相关文献

商品

酵母转化简介

转化是将外源DNA引入细胞，引起遗传变异或基因修饰的过程。1928年，Griffith首次报道了利用肺炎链球菌进行的转化。Avery等人于1944年证明了DNA转化的原理。

Yeast Transformation Introduction

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

实验方案

酵母转化实验方法

酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门