S2015
SSPE 缓冲液 20× 浓缩液
for Northern and Southern blotting, solution
别名:
20X SSPE Buffer, SSPE hybridization buffer, Saline-sodium phosphate-EDTA buffer
产品名称
SSPE 缓冲液 20× 浓缩液, for Northern and Southern blotting, solution
grade
Molecular Biology
sterility
0.2 μm filtered
form
solution
foreign activity
DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected
storage temp.
room temp
Quality Level
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Application
适用于：
- Northern 和 Southern 印迹杂交缓冲液
- 微阵列的清洗缓冲液
Features and Benefits
- 方便的即用型液体
- 杂交中的多用途
General description
SSPE Buffer 20x 液体是南方和北方杂交程序中的标准试剂。该液体可根据需要直接使用或稀释。
Preparation Note
SSPE Buffer 20x 在 0.2M 磷酸盐缓冲液 (pH 7.4) 中含有 0.02M EDTA 和 2.98M NaCl。
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Genome-wide analysis of barcoded Saccharomyces cerevisiae gene-deletion mutants in pooled cultures.
Pierce SE, et al.
Nature Protocols, 2, 2958-2974 (2007)
Glucocorticoid antagonist RU38486 fails to block acid-induced muscle wasting in vivo or in vitro.
Pickering WP, et al.
Nephrology, Dialysis, and Transplantation, 18, 1475-1484 (2003)
Warren P Pickering et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 18(8), 1475-1484 (2003-08-05)
Increased protein degradation during metabolic acidosis contributes to muscle wasting in uraemia. Adrenalectomy experiments in severely acidotic rats (arterial pH approximately 7.15) have shown that this is prevented in the absence of glucocorticoid. It should therefore be possible to block
Claudia S Meier-Trummer et al.
PloS one, 4(7), e6265-e6265 (2009-07-16)
Malignant catarrhal fever (MCF) is a lethal disease of cattle, characterized by vasculitis, necrosis, and accumulation of activated, dysregulated cytotoxic lymphocytes in various tissues. Ovine gamma herpesvirus 2 (OvHV-2) is a causative agent of MCF, which may trigger the disease
实验方案
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
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