Quality Segment
form
liquid (2x concentrate)
technique(s)
nucleic acid detection: suitable
foreign activity
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
-10 to -25°C
General description
Sigma的预杂交液是一种2倍浓缩的即用型杂交缓冲液，适用于Northern和Southern印迹方案。
Application
适合用作Northern和Southern印迹方案的预杂交缓冲液。
Preparation Note
Sigma的2倍预杂交液含200 μg/mL DNA，溶剂为10倍SSC和2倍Denhardt溶液：
- 200 μg/mL DNA
- 2mg牛血清白蛋白(BSA)
- 2mg Ficoll
- 2mg聚乙烯吡咯烷酮(PVP)
- 0.15 M柠檬酸钠
- 1.5M NaCl
存储类别
10 - Combustible liquids
WGK
WGK 2
闪点 (F)
Not applicable
闪点 (°C)
Not applicable