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Merck
CN

C7594

Sigma-Aldrich

酪蛋白封闭缓冲液

for Northern and Southern blotting, powder blend

别名:

blocking buffer

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About This Item

EC 号:
UNSPSC代码:
12161700
NACRES:
NA.25

等级

for molecular biology

质量水平

形式

powder blend

技术

Southern blotting: suitable

pH值(酸碱度)

6.8-7.2(reconstituted per directions)

溶解性

H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)

储存温度

room temp

相关类别

一般描述

酪蛋白是一种牛奶来源的物质,通常用于 Western、Southern 和 Northern 印迹实验中的封闭缓冲液。封闭剂用于减少检测试剂与膜的非特异性结合。

应用

适合用作 Western、Southern 和 Northern 印迹膜的封闭缓冲液。然后,封闭膜可用于化学发光,比色或 DIG 检测分析。

特点和优势

  • 封闭性能优于奶粉或 BSA
  • 与中性和正电荷膜兼容
  • 与化学发光,比色和 DIG 检测分析兼容

制备说明

将瓶中的全部内容物溶解在 800 mL 分子生物学级水中。搅拌直至充分混合,然后加水至终体积 1L。在 2-8°C 下最多保存一周。

WGK

WGK 1

法规信息

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Tadatsura Koshika et al.
Immunology, 134(4), 386-397 (2011-11-03)
α1,3-Galactosyltransferase gene-knockout pigs transgenic for porcine cytotoxic T-lymphocyte antigen 4 immunoglobulin (pCTLA4-Ig) have been produced to reduce T-cell-mediated rejection following xenotransplantation. The level of soluble pCTLA4-Ig in their blood was greatly in excess of the therapeutic level in patients, rendering
Ochratoxin A in some French wines: application of a direct competitive ELISA based on an OTA-HRP conjugate.
Radoi, A., et al.
Analytical Letters, 42, 1187-1187 (2009)
Serapion Pyrpassopoulos et al.
Methods in molecular biology (Clifton, N.J.), 1046, 389-403 (2013-07-23)
Many proteins that reside in the cytoplasm bind directly to cell membranes and play roles in signaling, adhesion, metabolism, cell structure, and cell motility. Several of these membrane-binding proteins, especially cytoskeletal proteins, have mechanical functions that result in the transmission
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Elizabeth R Sunderhaus et al.
Frontiers in neuroscience, 13, 1207-1207 (2019-11-30)
Mutations in patatin-like phospholipase domain-containing protein 6 (PNPLA6) have been linked with a number of inherited diseases with clinical symptoms that include spastic paraplegia, ataxia, and chorioretinal dystrophy. PNPLA6 is an evolutionary conserved protein whose ortholog in Drosophila is Swiss-Cheese

实验方案

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

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