等级
for molecular biology
质量水平
形式
powder blend
技术
Southern blotting: suitable
pH值(酸碱度)
6.8-7.2(reconstituted per directions)
溶解性
H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)
储存温度
room temp
一般描述
酪蛋白是一种牛奶来源的物质,通常用于 Western、Southern 和 Northern 印迹实验中的封闭缓冲液。封闭剂用于减少检测试剂与膜的非特异性结合。
应用
适合用作 Western、Southern 和 Northern 印迹膜的封闭缓冲液。然后,封闭膜可用于化学发光,比色或 DIG 检测分析。
特点和优势
- 封闭性能优于奶粉或 BSA
- 与中性和正电荷膜兼容
- 与化学发光,比色和 DIG 检测分析兼容
制备说明
将瓶中的全部内容物溶解在 800 mL 分子生物学级水中。搅拌直至充分混合,然后加水至终体积 1L。在 2-8°C 下最多保存一周。
相关产品
产品编号
说明
价格
WGK
WGK 1
法规信息
监管及禁止进口产品
Immunology, 134(4), 386-397 (2011-11-03)
α1,3-Galactosyltransferase gene-knockout pigs transgenic for porcine cytotoxic T-lymphocyte antigen 4 immunoglobulin (pCTLA4-Ig) have been produced to reduce T-cell-mediated rejection following xenotransplantation. The level of soluble pCTLA4-Ig in their blood was greatly in excess of the therapeutic level in patients, rendering
Ochratoxin A in some French wines: application of a direct competitive ELISA based on an OTA-HRP conjugate.
Analytical Letters, 42, 1187-1187 (2009)
Methods in molecular biology (Clifton, N.J.), 1046, 389-403 (2013-07-23)
Many proteins that reside in the cytoplasm bind directly to cell membranes and play roles in signaling, adhesion, metabolism, cell structure, and cell motility. Several of these membrane-binding proteins, especially cytoskeletal proteins, have mechanical functions that result in the transmission
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Frontiers in neuroscience, 13, 1207-1207 (2019-11-30)
Mutations in patatin-like phospholipase domain-containing protein 6 (PNPLA6) have been linked with a number of inherited diseases with clinical symptoms that include spastic paraplegia, ataxia, and chorioretinal dystrophy. PNPLA6 is an evolutionary conserved protein whose ortholog in Drosophila is Swiss-Cheese
实验方案
Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门