推荐产品
等级
for molecular biology
质量水平
表单
powder blend
技术
Southern blotting: suitable
pH值（酸碱度）
6.8-7.2(reconstituted per directions)
溶解性
H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)
储存温度
room temp
一般描述
酪蛋白是一种牛奶来源的物质，通常用于 Western、Southern 和 Northern 印迹实验中的封闭缓冲液。封闭剂用于减少检测试剂与膜的非特异性结合。
应用
适合用作 Western、Southern 和 Northern 印迹膜的封闭缓冲液。然后，封闭膜可用于化学发光，比色或 DIG 检测分析。
特点和优势
- 封闭性能优于奶粉或 BSA
- 与中性和正电荷膜兼容
- 与化学发光，比色和 DIG 检测分析兼容
制备说明
将瓶中的全部内容物溶解在 800 mL 分子生物学级水中。搅拌直至充分混合，然后加水至终体积 1L。在 2-8°C 下最多保存一周。
相关产品
产品编号
说明
价格
储存分类代码
11 - Combustible Solids
WGK
WGK 1
法规信息
监管及禁止进口产品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Tadatsura Koshika et al.
Immunology, 134(4), 386-397 (2011-11-03)
α1,3-Galactosyltransferase gene-knockout pigs transgenic for porcine cytotoxic T-lymphocyte antigen 4 immunoglobulin (pCTLA4-Ig) have been produced to reduce T-cell-mediated rejection following xenotransplantation. The level of soluble pCTLA4-Ig in their blood was greatly in excess of the therapeutic level in patients, rendering
W Querbes et al.
Journal of virology, 80(19), 9402-9413 (2006-09-16)
Invasion of glial cells by the human polyomavirus, JC virus (JCV), leads to a rapidly progressing and uniformly fatal demyelinating disease known as progressive multifocal leukoencephalopathy. The endocytic trafficking steps used by JCV to invade cells and initiate infection are
Xiaoli Ma et al.
BMC biochemistry, 11, 37-37 (2010-09-24)
Minichromosome maintenance proteins (Mcm) 2, 3, 4, 5, 6 and 7 are related by sequence and form a variety of complexes that unwind DNA, including Mcm4/6/7. A Mcm4/6/7 trimer forms one half of the Mcm2-7 hexameric ring and can be
Seiichi Katayama et al.
Acta medica Okayama, 60(6), 351-355 (2006-12-27)
Clostridium perfringens is an anaerobic spore-forming pathogen of humans and animals. C. perfringens type A strains, 13, CPN50, and NCTC8237, isolated from human gas gangrene, bound specifically to human fi bronectin (Fn). The trypsin-treatment of the bacterial cells significantly reduced
Serapion Pyrpassopoulos et al.
Methods in molecular biology (Clifton, N.J.), 1046, 389-403 (2013-07-23)
Many proteins that reside in the cytoplasm bind directly to cell membranes and play roles in signaling, adhesion, metabolism, cell structure, and cell motility. Several of these membrane-binding proteins, especially cytoskeletal proteins, have mechanical functions that result in the transmission
实验方案
Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
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