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Merck
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安全信息

B6429

Sigma-Aldrich

酪蛋白封闭缓冲液 10x

for Northern and Southern blotting, solution

别名:

10x Blocking Buffer

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About This Item

UNSPSC代码:
12161700
NACRES:
NA.54

等级

for molecular biology

质量水平

形式

solution

技术

Southern blotting: suitable

储存温度

room temp

相关类别

一般描述

酪蛋白是存在于乳汁中的磷蛋白。它分为四种亚型,包括αS1-、αS2-、β-和 κ-酪蛋白。
Sigma 的 10x 封闭缓冲液是一种基于酪蛋白的封闭剂,常规用于减少检测试剂与 Northern 和 Southern 印迹膜的非特异性结合。

应用

酪蛋白封闭缓冲液10X已用于:

  • 蛋白印迹
  • 酶联免疫吸附分析(ELISA)
  • 免疫组化

特点和优势

  • 封闭性能优于奶粉或 BSA
  • 易于使用的浓缩液
  • 与中性和正电荷膜兼容
  • 与化学发光和比色检测试剂盒兼容

制备说明

使用前用分子生物学级水稀释 10x 封闭缓冲液。

象形图

Exclamation markEnvironment

警示用语:

Warning

危险分类

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Some 30 years after its discovery, phage display remains one of the most widely used methods of in vitro selection. Initially developed to revolutionize the generation of therapeutic antibodies, phage display is now the first choice for screening artificial binding

实验方案

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

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