一般描述
酪蛋白是存在于乳汁中的磷蛋白。它分为四种亚型,包括αS1-、αS2-、β-和 κ-酪蛋白。
Sigma 的 10x 封闭缓冲液是一种基于酪蛋白的封闭剂,常规用于减少检测试剂与 Northern 和 Southern 印迹膜的非特异性结合。
Sigma 的 10x 封闭缓冲液是一种基于酪蛋白的封闭剂,常规用于减少检测试剂与 Northern 和 Southern 印迹膜的非特异性结合。
应用
酪蛋白封闭缓冲液10X已用于:
- 蛋白印迹
- 酶联免疫吸附分析(ELISA)
- 免疫组化
特点和优势
- 封闭性能优于奶粉或 BSA
- 易于使用的浓缩液
- 与中性和正电荷膜兼容
- 与化学发光和比色检测试剂盒兼容
制备说明
使用前用分子生物学级水稀释 10x 封闭缓冲液。
推荐
产品编号
说明
价格
警示用语:
Warning
危险分类
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
监管及禁止进口产品
Choline availability modulates human neuroblastoma cell proliferation and alters the methylation of the promoter region of the cyclin-dependent kinase inhibitor 3 gene
Journal of Neurochemistry, 89(5), 1252-1259 (2004)
American journal of physiology. Regulatory, integrative and comparative physiology, 302(2), R300-R311 (2011-10-07)
Claudins are the major determinants of paracellular epithelial permeability in multicellular organisms. In Atlantic salmon (Salmo salar L.), we previously found that mRNA expression of the abundant gill-specific claudin 30 decreases during seawater (SW) acclimation, suggesting that this claudin is
The journal of allergy and clinical immunology. In practice, 8(9), 3084-3092 (2020-05-12)
Fish collagen is widely used in medicine, cosmetics, and the food industry. However, its clinical relevance as an allergen is not fully appreciated. This is likely due to collagen insolubility in neutral aqueous solutions, leading to low abundance in commercially
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 20(1), 43-49 (2006-01-06)
The availability of choline during critical periods of fetal development alters hippocampal development and affects memory function throughout life. Choline deficiency during fetal development reduces proliferation and migration of neuronal precursor cells in the mouse fetal hippocampus and these changes
Isolation of isoform-specific binding proteins (Affimers) by phage display using negative selection.
Science signaling, 10(505) (2017-11-16)
Some 30 years after its discovery, phage display remains one of the most widely used methods of in vitro selection. Initially developed to revolutionize the generation of therapeutic antibodies, phage display is now the first choice for screening artificial binding
实验方案
Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门