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M5755

Sigma-Aldrich

MOPS-EDTA-乙酸钠 (MESA)

powder for RNA electrophoresis buffers

别名:

MESA缓冲液

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About This Item

UNSPSC代码:
12161700
NACRES:
NA.25

等级

for molecular biology

质量水平

200

产品线

BioReagent

表单

powder

技术

electrophoresis: suitable

pH值（酸碱度）

6.8-7.2(reconstituted per directions)

溶解性

H2O: soluble, faintly yellow

异质活性

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

储存温度

room temp

相关类别

一般描述

MOPS-EDTA-乙酸钠（MESA）是Northern印迹程序之前RNA电泳最常用的运行缓冲液。 对于最大值分离度，建议施加5 V/cm的电压。

应用

适合在制备甲醛-琼脂糖凝胶和RNA电泳相关的运行缓冲液时使用。

组分

1x MESA 缓冲液包含：
  • 40 mM MOPS
  • 10 mM乙酸钠
  • 1 mM EDTA （pH 8.3）

重悬

将瓶中的全部内容物溶解在最终体积为1L的分子生物学级水中。 这产生了10X MESA贮备液，可以根据需要进一步稀释。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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分析证书(COA)

Lot/Batch Number
0000383887
0000257463
0000344083
0000257463
0000344083

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Kathryn M Jones et al.
Journal of bacteriology, 184(9), 2491-2499 (2002-04-12)
Two operons have been cloned from Anabaena sp. strain PCC 7120 DNA, each of which encodes the three core subunits of distinct mitochondrial-type cytochrome c oxidases. The two operons are only 72 to 85% similar to one another at the
Molecular techniques for detection of gene expression in neuroimmunology.
Cowan, E.P., and Dhib-Jalbut, S.S.
Methods in Neuroscience, 24, 41-41 (1995)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 7-7 (1989)
Xueyu Xiang et al.
Developmental biology, 275(2), 343-355 (2004-10-27)
Recently, we cloned and sequenced the cDNA of allurin, a sperm chemoattractant isolated from the jelly of Xenopus laevis eggs [Proc. Natl. Acad. Sci. U.S.A. 78 (2001) 11205]. In this report, we demonstrate that allurin mRNA is expressed almost exclusively
Electrophoresis in agarose and acrylamide gels.
R C Ogden et al.
Methods in enzymology, 152, 61-87 (1987-01-01)
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