等级
for molecular biology
质量水平
产品线
BioReagent
形式
powder
技术
electrophoresis: suitable
pH值（酸碱度）
6.8-7.2(reconstituted per directions)
溶解性
H2O: soluble, faintly yellow
异质活性
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
储存温度
room temp
一般描述
MOPS-EDTA-乙酸钠（MESA）是Northern印迹程序之前RNA电泳最常用的运行缓冲液。 对于最大值分离度，建议施加5 V/cm的电压。
应用
适合在制备甲醛-琼脂糖凝胶和RNA电泳相关的运行缓冲液时使用。
组分
1x MESA 缓冲液包含：
- 40 mM MOPS
- 10 mM乙酸钠
- 1 mM EDTA （pH 8.3）
重悬
将瓶中的全部内容物溶解在最终体积为1L的分子生物学级水中。 这产生了10X MESA贮备液，可以根据需要进一步稀释。
相关产品
产品编号
说明
价格
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Sambrook, J., et al.
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Molecular techniques for detection of gene expression in neuroimmunology.
Cowan, E.P., and Dhib-Jalbut, S.S.
Methods in Neuroscience, 24, 41-41 (1995)
Kathryn M Jones et al.
Journal of bacteriology, 184(9), 2491-2499 (2002-04-12)
Two operons have been cloned from Anabaena sp. strain PCC 7120 DNA, each of which encodes the three core subunits of distinct mitochondrial-type cytochrome c oxidases. The two operons are only 72 to 85% similar to one another at the
Xueyu Xiang et al.
Developmental biology, 275(2), 343-355 (2004-10-27)
Recently, we cloned and sequenced the cDNA of allurin, a sperm chemoattractant isolated from the jelly of Xenopus laevis eggs [Proc. Natl. Acad. Sci. U.S.A. 78 (2001) 11205]. In this report, we demonstrate that allurin mRNA is expressed almost exclusively
Sheetal Sharma et al.
Cell reports, 34(1), 108565-108565 (2021-01-07)
The MRE11-RAD50-NBS1 (MRN) complex supports the synthesis of damage-induced long non-coding RNA (dilncRNA) by RNA polymerase II (RNAPII) from DNA double-strand breaks (DSBs) by an unknown mechanism. Here, we show that recombinant human MRN and native RNAPII are sufficient to
实验方案
TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
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