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安全信息

D4522

Sigma-Aldrich

脱氧核糖核酸 来源于小牛胸腺

Type XV, Activated, lyophilized powder

别名:

DNA 来源于小牛胸腺, 小牛胸腺DNA, 胸腺核酸

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About This Item

CAS号:
91080-16-9
EC 号:
293-507-3
MDL编号:
MFCD00151698
UNSPSC代码:
41106310
eCl@ss:
32160414
NACRES:
NA.52

等级

for molecular biology

质量水平

200

类型

Type XV

形式

lyophilized powder

储存温度

−20°C

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相关类别

一般描述

从雄性和雌性小牛的胸腺分离的高质量双链模板DNA。

应用

小牛胸腺DNA非常适合用于作为DNA聚合酶测定的底物、长片段的扩增、以及作为沉淀时的载体DNA。它被用作确定凝胶中DNA含量的标准品 以及通过荧光检测对DNA进行定量。
小牛胸腺DNA非常适合用于作为DNA聚合酶测定的底物、长片段的扩增、以及作为沉淀时的载体DNA。

特点和优势

•在37°C温育16小时后未检测到核酸酶活性
•高质量的模板DNA

数量

1mg DNA约为20个 A260 单位。

制备说明

使用Aposhian和Kornberg的方法，从高度聚合的小牛胸腺DNA中进行制备，并用DNase进行切口。

重悬

将DNA在分子生物级水中溶解过夜，终浓度为1mg/ml。

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储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

法规信息

动植物源性产品

分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is Product D4522, Deoxyribonucleic acid, single or double stranded?

    Even though it is activated or "nicked" DNA, we have found that it is primarily double stranded.

  6. What is the solution stability of Product D4522, Deoxyribonucleic acid?

    Solutions of deoxyribonucleic acid have been stored successfully for several months at 4°C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent.  At low concentrations (25 μg/mL), DNA tends to absorb onto the surfaces of plastic tubes.  It is not recommended to store deoxyribonucleic acid in alkaline solutions since it tends to degrade at pH greater than 8.0.  

  7. What is the molecular weight of Product D4522, Deoxyribonucleic acid?

    The molecular weight is reported to be between 10-15 million Daltons prior to activation (nicking).

  8. Is BSA (bovine serum albumin) used during the method of preparation for Product D4522, Deoxyribonucleic acid?

    During the method of preparation, when deoxyribonucleic acid is nicked, BSA is added to the solution.  This is followed by an extraction method to remove all exogenous protein. We do not, however, claim this deoxyribonucleic acid is 100% clean of all proteins (including BSA).

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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