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S8140

Sigma-Aldrich

SSPE Buffer

for Northern and Southern blotting, powder blend

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别名:
Saline-Sodium Phosphate-EDTA Buffer
UNSPSC代码:
41105319
NACRES:
NA.25

等级

for molecular biology

质量水平

200

形式

powder blend

pH值（酸碱度）

7.2-7.6 (20 × in water, high purity, ambient)

溶解性

water, high purity: 20 ×, clear, colorless

异质活性

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

储存温度

room temp

相关类别

一般描述

SSPE Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. This powder should be reconstituted to the indicated volume with molecular biology grade water before use. The resulting 20x liquid can be used straight or diluted as needed.

应用


  • Influence of Saline Buffers over the Stability of High-Annealed Gold Nanoparticles Formed on Coverslips for Biological and Chemosensing Applications.: This study explores the role of saline buffers in maintaining the stability of high-annealed gold nanoparticles used in biological and chemosensing applications. The findings highlight the potential for enhanced sensor performance in detecting biological molecules (Zhou & Ionescu, 2020).

  • Quantification of Surface Etching by Common Buffers and Implications on the Accuracy of Label-Free Biological Assays.: This research assesses the impact of various buffers, including SSPE buffer, on the surface etching of substrates used in label-free biological assays. The results indicate that buffer-induced surface changes can significantly affect assay accuracy (Ahn et al., 2012).

特点和优势

  • Easy to reconstitute powder
  • Multi-purpose use in hybridizations

组分

Final SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Microfluidic-based enzymatic on-chip labeling of miRNAs
Sonja Vorwek
New Biotechnology (2008)
3'-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures
Igor V. Kutyavin
Nucleic Acids Research, 28(2) (2000)

实验方案

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

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