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B6185

Sigma-Aldrich

Bionic™ Buffer, 10X Concentrate

for Northern and Southern blotting, solution

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别名:
Sodium Borate Electrophoresis Buffer
UNSPSC代码:
41105319
NACRES:
NA.25

等级

for molecular biology

质量水平

200

描述

Mode of use Voltage of 150 V is recommended for small electrophoresis chambers while a mid to large apparatus may be run at 200-250 V. User should test their chamber to determine optimum voltage.

形式

solution

浓度

10 ×

杂质

DNase, RNase and nickase, none detected

pH值（酸碱度）

8.0-8.5 (20-25 °C)

储存温度

room temp

相关类别

一般描述

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.

应用

Suitable for:
  • Use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures
  • Use with TBA and TAE gels
  • Preparation of agarose gels

特点和优势

  • Faster run times
  • Sharper bands
  • Easy to dilute and use
  • Flexible use with a variety of gel types

包装

The 4 L and 10 L sizes are supplied in dispensers with a spigot.

注意

Test electrophoresis chambers to determine highest voltage. Voltages beyond the recommended range for your apparatus may result in blown fuses or damage to your apparatus.

法律信息

Bionic is a trademark of Sigma-Aldrich Co. LLC

象形图

Health hazardExclamation mark
GHS08,GHS07

警示用语：

Danger

危险声明

H319,H360FD

危险分类

Eye Irrit. 2 - Repr. 1B

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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实验方案

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

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