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Merck
CN

B6185

Bionic™ Buffer, 10X Concentrate

for Northern and Southern blotting, solution

别名:

Sodium Borate Electrophoresis Buffer

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NACRES:
NA.25
UNSPSC Code:
41105319

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产品名称

Bionic™ Buffer, 10X Concentrate, for Northern and Southern blotting, solution

grade

Molecular Biology

description

Mode of use Voltage of 150 V is recommended for small electrophoresis chambers while a mid to large apparatus may be run at 200-250 V. User should test their chamber to determine optimum voltage.

form

solution

concentration

10 ×

impurities

DNase, RNase and nickase, none detected

pH

8.0-8.5 (20-25 °C)

storage temp.

room temp

Quality Level

200

相关类别

Packaging

The 4 L and 10 L sizes are supplied in dispensers with a spigot.

Legal Information

Bionic is a trademark of Sigma-Aldrich Co. LLC

Application

Suitable for:
  • Use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures
  • Use with TBA and TAE gels
  • Preparation of agarose gels

Disclaimer

Test electrophoresis chambers to determine highest voltage. Voltages beyond the recommended range for your apparatus may result in blown fuses or damage to your apparatus.

Features and Benefits

  • Faster run times
  • Sharper bands
  • Easy to dilute and use
  • Flexible use with a variety of gel types

General description

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B

存储类别

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000371239
0000371237
0000371239
0000371237
0000279962

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实验方案

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

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