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25 G
¥1,660.00
100 G
¥4,180.06
About This Item
¥1,660.00
请联系客服了解存货情况
推荐产品
生物来源
bovine thymus
质量水平
等级
Molecular Biology
方案
9-11 mg/mL (DNA concentration)
表单
solution
运输
dry ice
储存温度
−20°C
1 of 4
此商品 | PHR1383 | A8626 | A2786 |
|---|---|---|---|
| technique(s) cell culture | plant: suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) UV/Vis spectroscopy: suitable | technique(s) cell culture | mammalian: suitable |
| assay ≥99% | assay - | assay ≥99% | assay ≥99% |
| form powder | form - | form powder | form powder |
| application(s) agriculture | application(s) pharmaceutical (small molecule) | application(s) - | application(s) - |
| solubility 0.5 M HCl: soluble 20 mg/mL, clear to slightly hazy, colorless to faintly yellow | solubility - | solubility 0.5 M HCl: soluble 20 mg/mL, Grade III, colorless to faint yellow or tan | solubility 0.5 M HCl: soluble 20 mg/mL, clear to slightly hazy, colorless to faintly yellow |
| mp >360 °C (lit.) | mp >360 °C (lit.) | mp >360 °C (lit.) | mp >360 °C (lit.) |
一般描述
A ready-to-use solution of high quality double-stranded template DNA isolated from the thymus of male and female calves. The solution is supplied at a concentration of 9 - 12 mg/ml .
应用
Deoxyribonucleic acid solution from calf thymus is suitable for use as a blocking agent in Southern hybridizations. It was used as negative control in DNA-DNA hybridization experiments using genomic DNA preparations of Vibrio parahaemolyticus and Vibrio alginolyticus.[1] It was used as a standard for DNA quantification by fluorescent assay.[2]
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
Deoxyribonucleic acid solution from calf thymus has been used in in situ hybridization[3][4] and microarray hybridization.[2]
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
Deoxyribonucleic acid solution from calf thymus has been used in in situ hybridization[3][4] and microarray hybridization.[2]
Sigma offers sonicated, denatured DNA as an aqueous solution, at a concentration of 10 mg per mL, for use as a blocking agent in Northern and Southern blotting.
制备说明
This DNA is phenol-chloroform extracted, ethanol precipitated, and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.
其他说明
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
相关产品
产品编号
说明
价格
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Annick Robert-Pillot et al.
FEMS microbiology letters, 215(1), 1-6 (2002-10-24)
We compared the efficiencies of biochemical methods and polymerase chain reaction (PCR) for the identification of Vibrio parahaemolyticus strains. The 122 isolates studied, identified by biochemical tests as V. parahaemolyticus or Vibrio alginolyticus, were tested by R72H PCR assay. The
C Booth et al.
Nucleic acids research, 29(21), 4414-4422 (2001-11-03)
We have developed a method that allows quantitative amplification of single-stranded DNA (QAOS) in a sample that is primarily double-stranded DNA (dsDNA). Single-stranded DNA (ssDNA) is first captured by annealing a tagging primer at low temperature. Primer extension follows to
Lawrence E Heisler et al.
Nucleic acids research, 33(9), 2952-2961 (2005-05-25)
An effective tool for the global analysis of both DNA methylation status and protein-chromatin interactions is a microarray constructed with sequences containing regulatory elements. One type of array suited for this purpose takes advantage of the strong association between CpG
William D Hardie et al.
American journal of respiratory cell and molecular biology, 37(3), 309-321 (2007-05-15)
Expression of transforming growth factor alpha (TGF-alpha) in the respiratory epithelium of transgenic mice caused pulmonary fibrosis, cachexia, pulmonary hypertension, and altered lung function. To identify genes and molecular pathways mediating lung remodeling, mRNA microarray analysis was performed at multiple
Clare L Abram et al.
The Journal of biological chemistry, 278(19), 16844-16851 (2003-03-05)
Fish is a scaffolding protein and Src substrate. It contains an amino-terminal Phox homology (PX) domain and five Src homology 3 (SH3) domains, as well as multiple motifs for binding both SH2 and SH3 domain-containing proteins. We have determined that
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