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质量水平
用途
sufficient for 10-20 g (animal tissue)
sufficient for 50 assays (2 mL)
保质期
2 yr
技术
fractionation: suitable
运输
dry ice
储存温度
−20°C
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应用
- 分离线粒体和胞浆组分,用于测定支链氨基酸转氨酶(BCAT)活性
- 分离肾小球和心脏线粒体
- 分离脑损伤样品中的线粒体,检测线粒体跨膜电位
特点和优势
- 特制提取剂和可靠实验方案适合少量分离——在离心管中即可分离富集完整线粒体组分。
- 获得完整有活性的线粒体——所得线粒体适用于细胞凋亡、氧化应激等体外分析研究。
- 提供可确定内膜完整性的染色剂和实验方案——无需购买其他试剂,即可确定线粒体内膜完整性。
- 适配细胞色素C氧化酶检测试剂盒——可轻松确定线粒体外膜完整性。
试剂盒组分也可单独购买
- T9201Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solid化学品安全说明书
相关产品
警示用语:
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
10 - Combustible liquids
法规信息
从最新的版本中选择一种:
分析证书(COA)
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
Can Product MITOISO1, Mitochondria Isolation Kit, be used for isolation of mitochondria from cultured cells?
It is possible to use this kit for cultured cells, however MITOISO2 is a kit that was specifically designed for this purpose. This kit was originally designed for isolation from tissues.
Are the extraction buffers from Product MITOISO1, Mitochondria Isolation Kit, available separately?
The extraction buffers supplied with this kit are only available with the kit.
When using Product MITOISO1, Mitochondria Isolation Kit, how long can I store my isolated mitochondria?
If only interested in the proteins isolated from mitochondria, storage at -20 deg C is recommended. If intact mitochondria are required, storage at 4 deg C is recommended. These intact mitochondria are stable after refrigeration for up to 24 hours.
How much tissue can I use with Product MITOISO1, Mitochondria Isolation Kit,?
The procedures described in the bulletin are for small amounts of tissue samples (50-200 mg). Larger amounts of tissue can be purified with modifications listed in the technical bulletin. We recommend a minimum of 50 mg.
Is the Product MITOISO1, Mitochondria Isolation Kit, JC-1 stain available separately?
Yes, while the component J4519 is not available separately, JC-1 stain is listed separately as Sigma Product No. T4069 or Product No. 40882.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
When using Product MITOISO2, Mitochondria Isolation Kit, how can I store my isolated mitochondria?
Please see below for information on how best to store your isolated mitochondria using the MITOISO2 kit:If only interested in the proteins isolated from mitochondria, storage at -20 deg C is recommended. If intact mitochondria are required, storage at 4 deg C is recommended. These intact mitochondria are stable after refrigeration for up to 24 hours.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
亚细胞组分离心分离是一种常用技术,广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同,因此通过离心,可从温和均质的样品中轻松分离和纯化细胞器组分。
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
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