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安全信息

MITOISO1

Sigma-Aldrich

线粒体分离试剂盒

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

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EC 号:
232-650-8
UNSPSC代码:
12352200
NACRES:
NA.32

质量水平

200

用途

sufficient for 10-20 g (animal tissue)
 sufficient for 50 assays (2 mL)

保质期

2 yr

技术

fractionation: suitable

运输

dry ice

储存温度

−20°C

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应用

线粒体分离试剂盒可简单、快速地分离动物组织中富集的线粒体组分，并检测线粒体内膜的电化学质子梯度(ΔΨ)。用于线粒体介导的细胞凋亡研究。
线粒体分离试剂盒已用于：
  • 分离线粒体和胞浆组分，用于测定支链氨基酸转氨酶(BCAT)活性
  • 分离肾小球和心脏线粒体
  • 分离脑损伤样品中的线粒体，检测线粒体跨膜电位

特点和优势

  • 特制提取剂和可靠实验方案适合少量分离——在离心管中即可分离富集完整线粒体组分。
  • 获得完整有活性的线粒体——所得线粒体适用于细胞凋亡、氧化应激等体外分析研究。
  • 提供可确定内膜完整性的染色剂和实验方案——无需购买其他试剂，即可确定线粒体内膜完整性。
  • 适配细胞色素C氧化酶检测试剂盒——可轻松确定线粒体外膜完整性。

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • T9201Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solid化学品安全说明书

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象形图

Health hazardExclamation mark
GHS08,GHS07

警示用语：

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 3

法规信息

新产品

分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. Can Product MITOISO1, Mitochondria Isolation Kit, be used for isolation of mitochondria from cultured cells?

    It is possible to use this kit for cultured cells, however MITOISO2 is a kit that was specifically designed for this purpose.  This kit was originally designed for isolation from tissues.

  3. Are the extraction buffers from Product MITOISO1, Mitochondria Isolation Kit, available separately?

    The extraction buffers supplied with this kit are only available with the kit.

  4. When using Product MITOISO1, Mitochondria Isolation Kit, how long can I store my isolated mitochondria?

    If only interested in the proteins isolated from mitochondria, storage at  -20 deg C is recommended.  If intact mitochondria are required, storage at 4 deg C is recommended.  These intact mitochondria are stable after refrigeration for up to 24 hours.

  5. How much tissue can I use with Product MITOISO1, Mitochondria Isolation Kit,?

    The procedures described in the bulletin are for small amounts of tissue samples (50-200 mg). Larger amounts of tissue can be purified with modifications listed in the technical bulletin.  We recommend a minimum of 50 mg.

  6. Is the Product MITOISO1, Mitochondria Isolation Kit,  JC-1 stain available separately?

    Yes, while the component J4519 is not available separately, JC-1 stain is listed separately as Sigma Product No. T4069 or Product No. 40882.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. When using Product MITOISO2, Mitochondria Isolation Kit, how can I store my isolated mitochondria?

    Please see below for information on how best to store your isolated mitochondria using the MITOISO2 kit:If only interested in the proteins isolated from mitochondria, storage at  -20 deg C is recommended.  If intact mitochondria are required, storage at 4 deg C is recommended.  These intact mitochondria are stable after refrigeration for up to 24 hours.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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