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质量水平
用途
sufficient for 50 applications (2-5 x 107 cells)
技术
ELISA: suitable
western blot: suitable
运输
dry ice
储存温度
−20°C
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一般描述
Mitochondria, a double membrane structure, is the site of most energy production in eukaryotic cells. The mitochondria isolation kit contains two buffers: one for extracting proteins for functional studies and another for profiling in proteome studies. Additionally, the kit includes a storage buffer for preserving intact mitochondria.
应用
线粒体分离试剂盒用于分离下列来源中的线粒体:
- Ewing′肉瘤肿瘤家族(ESFT)细胞
- 肺结核患者血液样本
- 人神经母细胞瘤细胞和神经胶质瘤细胞
- 肌小管
- HepG2细胞
特点和优势
- The kit allows for quick and simple extraction of a concentrated portion of mitochondria from cells.
- The isolated mitochondria will mostly have intact inner and outer membranes.
- Can be used to extract mitochondrial proteins for proteome analysis
其他说明
- This product is for R&D use only, not for drug, household, or other uses.
- Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
- Use ultrapure water for the preparation of reagents.
- Please refer to the product information sheet to learn more about kit components, reagents required, storage and stability, and isolation protocols.
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
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价格
警示用语:
Warning
危险分类
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2
WGK
WGK 3
闪点(°F)
closed cup
闪点(°C)
closed cup
法规信息
常规特殊物品
Mitochondria
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商品
亚细胞组分离心分离是一种常用技术,广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同,因此通过离心,可从温和均质的样品中轻松分离和纯化细胞器组分。
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。
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