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Merck
CN

MITOISO2

Sigma-Aldrich

线粒体分离试剂盒

sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

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别名:
Mitochondrial Extraction Kit, Mitochondrial Purification Kit
UNSPSC代码:
12352200
NACRES:
NA.32

质量水平

用途

sufficient for 50 applications (2-5 x 107 cells)

技术

ELISA: suitable
western blot: suitable

运输

dry ice

储存温度

−20°C

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一般描述

Mitochondria, a double membrane structure, is the site of most energy production in eukaryotic cells. The mitochondria isolation kit contains two buffers: one for extracting proteins for functional studies and another for profiling in proteome studies. Additionally, the kit includes a storage buffer for preserving intact mitochondria.

应用

线粒体分离试剂盒用于分离下列来源中的线粒体:
  • Ewing′肉瘤肿瘤家族(ESFT)细胞
  • 肺结核患者血液样本
  • 人神经母细胞瘤细胞和神经胶质瘤细胞
  • 肌小管
  • HepG2细胞

特点和优势

  • The kit allows for quick and simple extraction of a concentrated portion of mitochondria from cells.
  • The isolated mitochondria will mostly have intact inner and outer membranes.
  • Can be used to extract mitochondrial proteins for proteome analysis

其他说明

  • This product is for R&D use only, not for drug, household, or other uses.
  • Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
  • Use ultrapure water for the preparation of reagents.
  • Please refer to the product information sheet to learn more about kit components, reagents required, storage and stability, and isolation protocols.

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • C2978CelLytic M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 10 mL化学品安全说明书

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 2.5 mL化学品安全说明书

  • C0356Protein Extraction Reagent Type 4 1 bottle化学品安全说明书

警示用语:

Warning

危险分类

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2

WGK

WGK 3

闪点(°F)

closed cup

闪点(°C)

closed cup

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

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Mitochondria
Cooper G M
Journal of biological and chemical chronicles (2000)
Youguang Gao et al.
Journal of translational medicine, 18(1), 114-114 (2020-03-07)
We have reported that polydatin (PD) alleviates mitochondrial dysfunction in rat models of sepsis-induced acute kidney injury (SI-AKI), but the mechanism is not well understood. Here, we investigated the role of Parkin-mediated mitophagy in the protective effects of PD in
Vladislav V Glinsky et al.
Neoplasia (New York, N.Y.), 11(9), 901-909 (2009-09-03)
At present, there is no efficient curative therapy for cancer patients with advanced metastatic disease. Targeting of antiapoptotic molecules acting on the mitochondrial apoptosis pathway could potentially augment antimetastatic effect of cytotoxic drugs. Similarly to Bcl-2 family members, beta-galactoside-binding lectin
Theodora Panagaki et al.
Biomolecules, 10(4) (2020-04-29)
Down syndrome (trisomy of human chromosome 21) is a common genetic disorder. Overproduction of the gaseous mediator hydrogen sulfide (H2S) has been implicated in the pathogenesis of neurological and metabolic deficits associated with Down syndrome. Several lines of data indicate
Detection and characterisation of multi-drug resistance protein 1 (MRP-1) in human mitochondria
Roundhill, EA and Burchill, SA
British Journal of Cancer, 106(6), 1224-1224 (2012)

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