推荐产品
产品名称
Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
生物来源
bovine lung
质量水平
表单
DMSO solution
储存温度
−20°C
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一般描述
特异性
应用
抵御细胞和组织提取物的内源性酶活性
生化/生理作用
特点和优势
稳定储存：在DMSO中作为溶液供应，允许在-20°C下长期储存
性能恒定：即用型解决方案
组分
抑肽酶
乌苯美司
E-64
亮抑酶肽
抑肽素A
注意
制备说明
相关产品
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
188.6 °F - closed cup
闪点(°C)
87 °C - closed cup
法规信息
历史批次信息供参考:
分析证书(COA)
Is there anything in Product P8340, Protease Inhibitor Cocktail, that will cause downstream problems with RT-PCR?
While the product should not cause any downstream problems with RT-PCR, we would advise to clean up the reaction with either DMN10 (GenElute™ Direct mRNA Miniprep Kit) or T9424 (TRI Reagent®) for purification.
Can Product P8340, Protease Inhibitor Cocktail, be used with RIPA buffer and how much should be used?
Yes, this product can be used with RIPA buffer at 100 μl/ml concentration.
How much Product P8340, Protease Inhibitor Cocktail, should I use?
One mL of the cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g (wet weight) of bovine liver or 10 mL of cell lysate obtained from CHO cells at a cell density of 100 million (108) cells per mL. CHO cells were grown on DMEM with 10% FCS (heat inactivated). Note: Not all lysates contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.
What is the concentration of inhibitors in Product P8340, Protease Inhibitor Cocktail?
The concentration of inhibitors in this product is the following:AEBSF, 104 mMAprotinin, 0.085 mMBestatin, 4 mME-64, 1.4 mMLeupeptin, 2 mMPepstain A, 1.5 mM.
Does Product P8340, Protease Inhibitor Cocktail, contain any chelators or EDTA?
This product does not contain any metal chelators.
Can Product P8340, Protease Inhibitor Cocktail, be used with Ezview Protein A and Anti FLAG M2 affinity gels.
Yes, this product can be used with both Ezview Protein A and Anti FLAG M2 affinity gels.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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