General description
CelLytic M是一种专有的去污剂溶液，专为从培养的哺乳动物细胞中提取有效的全细胞蛋白而设计。 它能够对悬浮细胞和贴壁细胞进行有效和快速的细胞裂解和蛋白质溶解。 裂解物可用于许多下游应用而无需对CelLytic M进行去除，例如报告基因测定、Western印迹/免疫沉淀、凝胶阻滞实验、磷酸酶测定、以及激酶测定。
Features and Benefits
- 高效：效率比冻融、超声和其他产品最多高50％
- 非变性：不会干扰下游应用，如免疫沉淀、激酶和磷酸酶测定、报告基因检测、以及凝胶阻滞实验
- 方便：即用型试剂无需从培养板上刮下
- 快速：可在室温下快速裂解细胞。
Preparation Note
用于蛋白质提取的CelLytic M试剂的效率已经在但不限于HeLa、CHO、COS、HL-60、Jurkat、A431、PC-12和牛主动脉内皮细胞（BAEC）上进行了测试。
Analysis Note
106-107 悬浮细胞使用125μl CelLytic M 。 对于贴壁细胞，在100 mm平板中使用500-1,000μL；在35 mm平板中适用200-400μL。
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| C2978-250ML | 04061838445568 |
| C2978-40ML | 04061838248190 |
| C2978-50ML | 04061838445575 |
| C2978-10ML | 04061838248183 |
| C2978-60ML | 04061838112828 |