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Merck
CN
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C2978

Sigma-Aldrich

CelLytic™ M

Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.

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别名:
Whole-cell protein extraction solution
UNSPSC代码:
41116134
NACRES:
NA.56

质量水平

200

形式

solution

储存温度

room temp

相关类别

一般描述

CelLytic M是一种专有的去污剂溶液，专为从培养的哺乳动物细胞中提取有效的全细胞蛋白而设计。 它能够对悬浮细胞和贴壁细胞进行有效和快速的细胞裂解和蛋白质溶解。 裂解物可用于许多下游应用而无需对CelLytic M进行去除，例如报告基因测定、Western印迹/免疫沉淀、凝胶阻滞实验、磷酸酶测定、以及激酶测定。

特点和优势

  • 高效：效率比冻融、超声和其他产品最多高50％
  • 非变性：不会干扰下游应用，如免疫沉淀、激酶和磷酸酶测定、报告基因检测、以及凝胶阻滞实验
  • 方便：即用型试剂无需从培养板上刮下
  • 快速：可在室温下快速裂解细胞。

数量

用于蛋白质提取的CelLytic M试剂的效率已经在但不限于HeLa、CHO、COS、HL-60、Jurkat、A431、PC-12和牛主动脉内皮细胞（BAEC）上进行了测试。

分析说明

106-107 悬浮细胞使用125μl CelLytic M 。 对于贴壁细胞，在100 mm平板中使用500-1,000μL；在35 mm平板中适用200-400μL。

法律信息

CelLytic is a trademark of Sigma-Aldrich Co. LLC

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Will CelLytic™ M, Product No. C2978, interfere with protein concentration assays such as Bradford, Lowry and BCA?

    We have tested and found CelLytic™ M to be compatible for Bradford Assay and BCA assays.  For the Lowry method, there is a background issue that is problematic for low protein concentrations, but may be subtracted with high protein concentrations.

  2. Will Product No. C2978, CelLytic™ M solubilize membrane proteins?

    CelLytic™ M can solubilize membrane proteins as well as cytoplasmic proteins and the conditions are non-denaturing.  However, we cannot guarantee the solubilization of all membrane proteins. The following proteins were detected in the CelLytic™ M lysate when tested with these antibodies against membrane proteins: Placental alkaline phosphatase, CD45, Connexin and Caveolin.

  3. Is there any denaturants in Product C2978, CelLytic™ M that can interfere with my downstream applications?

    CelLytic™ M does not contain any primary amines; it is a buffered solution (pH 7.6) which has no denaturants except for a mild detergent.  The exact formulation is proprietary.  This product may be diluted or dialyzed if it is found to have interference in your particular assay.

  4. Does Product C2978, CelLytic™ M contain any protease inhibitors?

    No, CelLytic™ M does not contain protease inhibitors.  We recommend the addition of one of our protease inhibitor cocktails, such as Product No. P8340 for mammalian cells or Product No. P2714 for general use.

  5. Can Product C2978 CelLytic™ M be used for adherent cells?

    Yes, CelLytic™ M can be used for both adherent cells and cells in suspension. Use 125 μl CelLytic™ M for 106-107 of suspended cells. For adherent cells, use 500-1,000 μl for a 100 mm plate; 200-400 μl for a 35 mm plate.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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