推荐产品
应用
特点和优势
- 包括即用型试剂、色谱柱和 FLAG 融合蛋白亲和纯化的详细方案。
- ANTI-FLAG M2 亲和凝胶可有效结合 FLAG 融合蛋白，无需进行初步步骤和校准。
- 有效洗脱条件的两种选择（通过酸性条件或通过与 FLAG 肽竞争）。
包装
足以 3-5 次使用 1-ml 亲和纯化柱。
其他说明
试剂盒包含 CelLytic™M，用于哺乳动物细胞裂解和蛋白提取。
法律信息
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- C2978CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.化学品安全说明书
- SAE0194Purified 3xFLAG® peptide, ≥95% (HPLC), lyophilized powder化学品安全说明书
- A2220ANTI-FLAG® M2 Affinity Gel, purified immunoglobulin, buffered aqueous glycerol solution化学品安全说明书
- C2103Chromatography columns, general-purpose, volume 10 mL, Overall H 5 in.化学品安全说明书
储存分类代码
10 - Combustible liquids
法规信息
常规特殊物品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
M D Mostaqul Huq et al.
Molecular & cellular proteomics : MCP, 6(4), 677-688 (2007-01-09)
Retinoic acid receptors (RARs) belong to the nuclear receptor superfamily. The mechanism of ligand-dependent activation of RARs is well known. The effect of protein phosphorylation on the activity of RARs has also been demonstrated. However, it is unclear whether other
Takeshi Into et al.
Molecular and cellular biology, 28(4), 1338-1347 (2007-12-19)
Nitric oxide (NO) has been thought to regulate the immune system through S nitrosylation of the transcriptional factor NF-kappaB. However, regulatory effects of NO on innate immune responses are unclear. Here, we report that NO has a capability to control
Mayumi Kitagawa et al.
Cell reports, 7(1), 166-179 (2014-03-25)
The chromosome passenger complex (CPC) must relocate from anaphase chromosomes to the cell equator for successful cytokinesis. Although this landmark event requires the mitotic kinesin MKlp2, the spatiotemporal mechanistic basis remains elusive. Here, we show that phosphoregulation of MKlp2 by
Erik G Puffenberger et al.
PloS one, 7(1), e28936-e28936 (2012-01-27)
The Clinic for Special Children (CSC) has integrated biochemical and molecular methods into a rural pediatric practice serving Old Order Amish and Mennonite (Plain) children. Among the Plain people, we have used single nucleotide polymorphism (SNP) microarrays to genetically map
Amitava Mukherjee et al.
PLoS pathogens, 7(3), e1001311-e1001311 (2011-03-26)
The host innate immune response to viral infections often involves the activation of parallel pattern recognition receptor (PRR) pathways that converge on the induction of type I interferons (IFNs). Several viruses have evolved sophisticated mechanisms to attenuate antiviral host signaling
相关内容
用于纯化重组蛋白的蛋白纯化技术、试剂和方法包括离子交换、排阻层析和蛋白亲和层析。
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.
默克分享基因组学、克隆学和大量分子生物学技术的发展使研究人员能在众多生物系统中表达异源蛋白。表达重组蛋白的能力为研究人员提供了广泛而强大的下游应用空间，以便进一步开展研究。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门