FLAG® 标签蛋白纯化
FLAG® 标签使对重组融合蛋白进行精确检测和强力纯化成为可能，并在从结合到活性分析等众多下游应用中具有可靠功用。FLAG® 表位标签是一种短链亲水8-氨基酸肽（DYKDDDDK 标签），易于为肠激酶所剪切。由于FLAG® 标签尺寸小，且具有亲水特性，因此其通常依附于融合蛋白的表面，对融合蛋白的功能、分泌以及运输影响极小。不论您最终应用是什么，我们都有相关工具来表达、提纯和检测FLAG® 融合蛋白。
概要
Products
FLAG® 抗体选择指南
FLAG® 抗体结合特性
相关资源
- Brochure: Refine Protein Preparation
Today, researchers are challenged to create high quality samples for meaningful protein analysis, often using cumbersome traditional sample preparation methods. With over 50 years of experience in developing protein sample preparation technologies, Merck is constantly innovating new tools to offer you rapid and efficient solutions that can be smoothly integrated into your workflow.
- User Guide: FLAG - Proven System for Detection and Purification of Proteins
The FLAG Expression System is an established way to express, purify, and detect recombinant fusion proteins. FLAG and 3×FLAG® have proven utility in numerous applications such as Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. These small hydrophilic tags facilitate superior detection and purification of recombinant fusion proteins when using our highly specific and sensitive ANTI-FLAG® antibodies.
- Application Note: EZview™ Red Protein A and ANTI-FLAG® M2 Affinity Gels - Immunoprecipitation with Enhanced Visibility Affinity Beads
The strength and specificity of interactions between biomolecules have been exploited for years in biochemical research. Affinity-based protein purification techniques have been used extensively to isolate and purify specific proteins or classes of proteins from complex biochemical mixtures, such as cell lysates..
如要继续阅读，请登录或创建帐户。
暂无帐户？