推荐产品
一般描述
CelLytic™ MEM蛋白提取试剂盒提供了一种分离细胞中疏水性筏状微结构域相关蛋白的快速、方便的方法。该方法基于相分离，不需要分离细胞膜。分离的蛋白质可用于进一步的实验，例如 SDS-PAGE、蛋白质印迹、斑点印迹和免疫沉淀。 该试剂盒已在 HeLa、HEK-293、NIH 3T3、COS 和 CHO 及其它细胞系上进行了测试。
膜蛋白占据了生物体基因组大约20-30%的成分，充当着胞内的看门人、调节器和感应器的角色。它们具有多样的细胞功能，例如将细胞与外部的毒素隔离开来，作为胞内信号级联的起始点，以及维持临界离子浓度。
应用
CelLytic™ MEM 蛋白质提取试剂盒已被用于以肝细胞测定脂肪从头 合成和提取用于蛋白质印迹的膜蛋白。
适用性
提供足够进行80次检测的试剂。
法律信息
CelLytic is a trademark of Sigma-Aldrich Co. LLC
仅试剂盒组分
产品编号
说明
- Lysis and Separation Buffer 50 mL
- Wash Buffer for CelLytic MEM 50 mL
- Sodium Chloride, 4M Solution 1.5 mL
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 1 mL化学品安全说明书
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2
储存分类代码
10 - Combustible liquids
闪点(°F)
188.6 °F - closed cup
闪点(°C)
87 °C - closed cup
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Jun Yang et al.
Bioscience, biotechnology, and biochemistry, 83(6), 1117-1123 (2019-03-08)
It has been reported that lncRNA POU3F3 was upregulated in esophageal squamous-cell carcinomas, indicating its role as an oncogene in this disease. However, the mechanism of its function and its involvement in other malignancies is unknown. In the present study
Yue Zhao et al.
Cancer medicine, 7(8), 3977-3987 (2018-07-06)
Esophageal squamous cell carcinoma (ESCC) is a malignant disease with poor prognosis. Because of early metastasis prior to diagnosis and therapeutic resistance, ESCC has become one of the leading causes of cancer-related death. Here, we investigated the clinicopathological significance of
Amanda Y van Tilburg et al.
Microbial biotechnology, 15(5), 1633-1651 (2021-12-03)
A form of lateral membrane compartmentalization in bacteria is represented by functional membrane microdomains (FMMs). FMMs are important for various cellular processes and offer application possibilities in microbial biotechnology. We designed a lipidomics method to directly measure relative abundances of
Elisabeth P Carpenter et al.
Current opinion in structural biology, 18(5), 581-586 (2008-08-05)
Membrane protein structural biology is still a largely unconquered area, given that approximately 25% of all proteins are membrane proteins and yet less than 150 unique structures are available. Membrane proteins have proven to be difficult to study owing to
Hiroaki Tateno et al.
Glycobiology, 26(12), 1328-1337 (2016-04-03)
Human somatic stem cells such as human mesenchymal stem cells (hMSCs) are considered attractive cell sources for stem cell-based therapy. However, quality control issues have been raised concerning their safety and efficacy. Here we used lectin microarray technology to identify
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