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应用
特点和优势
- 比传统方法如超声法和溶菌酶法提取蛋白质效率高
- 可放大 1-25 克细菌细胞浆
- 不干扰下游应用，如亲和层析、IP 和免疫印迹
- 与蛋白酶抑制剂、抑制剂混合物、离液剂、盐、螯合剂、还原剂相容
其他说明
法律信息
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What bacterial cells can be used with CelLytic B?
CelLytic B Reagent by itself (no lysozyme or benzonase) was optimized for E. coli strain BL21. It also works with other common E. coli strains such as DH5?, JM109, and HB 101. The CelLytic B Reagent may be used on other similar bacterial cells. The CelLytic B Plus Kit is compatible with many more difficult to lyse Gram positive bacteria such as Bacillus and Streptococcus.
What is the difference between CelLytic B (Product Nos. B7435, B7310, C8740) and CelLytic B Plus (Product Nos. CB0050 and CB0500)?
CelLytic B Plus contains CelLytic B, Product No. B7435 as well as lysozyme, benzonase, and a protease inhibitor cocktail for a complete reagent for cell lysates for protein isolation.
What is the difference between CelLytic B (Product No. B7435), CelLytic B 2x (Product No. B7310) and CelLytic B 10x (Product No. C8740)?
CelLytic B 2X has double the strength of CelLytic B for small volume extractions. This allows CelLytic B 2X to be used when a higher protein concentration or lower volume is required. The original formula, CelLytic B, requires 10-20 ml of the reagent for 1 gram of wet cell paste. Only 5 ml of the CelLytic B 2X reagent is required to lyse and extract protein from 1 gram of wet cell paste. CelLytic B 10X is provided as a concentrated mixture of the proprietary detergents found in CelLytic B, without any buffering component. This allows the user to customize the extraction reagent by choosing the detergent concentration and buffering components ideal for the extraction and purification of the protein(s) of interest. CelLytic B 10X is also suitable for the lysis of yeast and mammalian cells.
Can I obtain protein from inclusion bodies with CelLytic B?
If the protein is found in inclusion bodies, you need to use an additional reagent, Product No. C5236, CelLytic IB to solubilize the proteins from the inclusion bodies. CelLytic B is used to isolate the inclusion bodies from the bacteria. CelLytic IB, Product No. C5236 can then be used to solubilize the proteins from the inclusion bodies.
Can I use the CelLytic™ B cell lysate in an activity assay?
CelLytic B contains a zwitterionic detergent so the protein is active and can be used in protein activity assays.
Can I concentrate my sample that was made using CelLytic B?
If concentrating a sample, be aware that concentration using filter concentrators may increase the detergent concentration significantly and cause issues in downstream applications. heoretically, ways to get around this are TCA precipitation, or diluting the extract below the CMC prior to concentration. This would be at least a 1/5 dilution, based on the following reference: Saito, S., and Tsuchiya, T., Characteristics of n-octyl beta-D-thioglucopyranoside, a new non-ionic detergent useful for membrane biochemistry. Biochem. J., 222(3), 829-832, 1984.
Can I use CelLytic B for eukaryotic cells?
CelLytic B was optimized for bacterial cells. It may lyse some mammalian cells. CelLytic B 10X is a better choice for the lysis of yeast and mammalian cells due to the increased detergent concentration.
Can I use CelLytic B with the maltose binding protein system (MBP System)?
CelLytic B cannot be used with the MBP system. We have used this in the maltose binding protein system, and know that it will NOT work in this system, since any and all detergents will destroy binding of protein to the resin.
When using the CelLytic™ B lysis reagent, do I need to make a dilution prior to measuring protein by Bradford assay?
When using the CelLytic™ B lysis reagent (B7435, B7310, C8740, and contained in kits CB0050 and CB0500), it is best to use a 1:10 dilution in order to reduce the risk of interference with the Bradford Reagent. A 1:20 dilution would be even better, as long as the protein level remains within the standard curve.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.
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