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70584-M

BugBuster^®蛋白提取试剂

Name: BugBuster<SUP>®</SUP>蛋白提取试剂
Brand: MM

别名:

Protein Extraction Reagent, Protein Extraction Solution

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About This Item

UNSPSC代码:

41106516

NACRES:

NA.32

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N,N′-二甲基乙二胺

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Sigma-Aldrich

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轮环藤宁

表单

liquid

质量水平

100

制造商/商品名称

Novagen^®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

储存温度

10-30°C

一般描述

BugBuster^®蛋白提取试剂是为温和地破坏大肠杆菌细胞壁并释放活性蛋白而配制的。它提供了一种简单、快速、低成本的替代机械方法，如弗氏压碎器或超声波法，用于释放表达的目标蛋白，以制备纯化或其他应用。该专利配方采用非离子和两性离子洗涤剂的三缓冲基混合物，能够在蛋白不变性的情况下完成细胞壁穿孔。BugBuster还可用于制备高纯度包涵体，用于表达的蛋白质不溶的情况下进行后续纯化。

应用

BugBuster^®蛋白提取试剂已用于：

裂解大肠杆菌BLR (DE3)细胞制备β-葡萄糖苷酶(BglB)并纯化
作为细胞裂解缓冲液，处理聚囊藻PCC6803转偶联物，并测定黄色荧光蛋白(YFP)的荧光
谷胱甘肽-转移酶-热休克蛋白家族A (Hsp70)成员8 (GST- HSPA8)的裂解缓冲液

警告

毒性：标准处理（A）

法律信息

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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Utilising the native plasmid, pCA2.4, from the cyanobacterium Synechocystis sp. strain PCC6803 as a cloning site for enhanced product production.

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The use of photosynthetic autotrophs and in particular the model organism Synechocystis PCC6803 is receiving much attention for the production of sustainable biofuels and other economically useful products through metabolic engineering. Optimisation of metabolic-engineered organisms for high-level sustained production of

Thermal stability and kinetic constants for 129 variants of a family 1 glycoside hydrolase reveal that enzyme activity and stability can be separately designed.

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PloS one, 12(5), e0176255-e0176255 (2017-05-23)

Accurate modeling of enzyme activity and stability is an important goal of the protein engineering community. However, studies seeking to evaluate current progress are limited by small data sets of quantitative kinetic constants and thermal stability measurements. Here, we report

Chaperone-mediated autophagy controls the turnover of E3 ubiquitin ligase MARCHF5 and regulates mitochondrial dynamics.

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Autophagy, 17(10), 2923-2938 (2021-05-11)

As a highly dynamic organelle, mitochondria undergo constant fission and fusion to change their morphology and function, coping with various stress conditions. Loss of the balance between fission and fusion leads to impaired mitochondria function, which plays a critical role

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Citation Spotlight: BugBuster^® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

BugBuster^® and Benzonase^® Reagents are the Clear Solutions to Simple, Efficient Extraction of E. coli Proteins

This article shows the use of BugBuster^® and Benzonase^® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield^® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield^® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

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