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一般描述
BugBuster®预混液可用于高效的蛋白纯化及其它相关应用。它可取代高压破菌(French press)或超声波处理等机械方法。提供两种包装规格，分别包含足以从20 g或100 g细胞糊中提取蛋白的试剂。
应用
BugBuster®预混液的应用实例：
- 在蛋白纯化实验中重悬和裂解细胞沉淀
- 提取免疫印迹用蛋白
- 作为细菌裂解缓冲液用于制备包涵体
生化/生理作用
BugBuster®蛋白提取试剂专门用于温和地破坏大肠杆菌细胞壁以释放可溶性蛋白。利用混合去垢剂的独特配方有效地穿破细胞壁但不会使可溶性蛋白变性。结合BugBuster试剂、rLysozyme溶液和Benzonase核酸酶，BugBuste预混液对蛋白的提取效率更高，获得的蛋白提取物下游处理更加方便。
特点和优势
- 为目标表达蛋白释放提供方便、快捷、高性价比的解决方案。
- 该预混液无需稀释或进行额外的分离操作。
- 革兰氏阳性和革兰氏阴性细菌的高效裂解
- 最大限度回收活性可溶性蛋白
- 能穿透细胞壁而不使可溶性蛋白变性
- 用于制备高纯度包涵体
- 与蛋白酶和磷酸酶抑制剂混合物兼容
警告
毒性：标准处理（A）
法律信息
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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商品
Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens
相关内容
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细胞裂解和蛋白质溶解是进行有效分析和高效处理的关键步骤，默克分享提取方法包括酶法、化学法、机械法或几种方法的联合使用。
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