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一般描述
BugBuster HT蛋白质提取试剂将BugBuster蛋白质提取试剂和Benzonase®核酸酶结合在一个方便的试剂中。BugBuster HT消除了传统细胞裂解程序引起的常见生物加工问题。从大肠杆菌中轻轻提取可溶性蛋白质,而无需暴露于热或氧化损伤,并且可以通过一步核酸消化消除粘度。所得的蛋白质提取物可以通过常规纯化技术容易地分级分离。BugBuster HT非常适合用于高通量蛋白质纯化。
应用
BugBuster® HT蛋白质提取试剂已用于:
- 在离心进行蛋白质表达和免疫印迹后裂解大肠杆菌细胞颗粒
- 从细菌细胞颗粒中提取蛋白质
- 裂解大肠杆菌细胞颗粒,用于表达和纯化dMIC60(线粒体内膜蛋白)蛋白
特点和优势
- 快速蛋白质提取和核酸降解
- 非常适合处理不同体积的各种样品
- 添加r溶菌酶溶液可确保提高提取率
警告
毒性:标准处理(A)
法律信息
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Chiara Pedicone et al.
iScience, 25(4), 104170-104170 (2022-04-26)
Here, we describe the use of artificial intelligence to identify novel agonists of the SH2-containing 5' inositol phosphatase 1 (SHIP1). One of the compounds, K306, represents the most potent agonist identified to date. We find that K306 exhibits selectivity for
商品
Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens
Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria using the BugBuster Plus Lysonase™ Kit
The combination of BugBuster® and Lysonase™ reagents greatly enhances the release of soluble proteins from Gram-positive bacteria.
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