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P8849

Sigma-Aldrich

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic, aminopeptidases and thermolysin-like activities, for use in purification of Histidine-tagged proteins, DMSO solution

别名:

Protease inhibitor solution

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About This Item

MDL编号:
MFCD00677817
UNSPSC代码:
12352200
NACRES:
NA.77
表单:
DMSO solution
储存温度:
−20°C

产品名称

Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solution

生物来源

synthetic

质量水平

300

表单

DMSO solution

储存温度

−20°C

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相关类别

一般描述

蛋白酶抑制剂的DMSO溶液专用于纯化组氨酸标签蛋白。

溶液中混有多种抑制剂成分，可特异性抑制丝氨酸、半胱氨酸、天冬氨酸、嗜热菌蛋白酶样蛋白酶和氨基肽酶。

特异性

抑制丝氨酸，半胱氨酸，天冬氨酸，和嗜热菌蛋白酶样蛋白酶和氨基肽酶。

应用

混合液经过专门的优化和验证，适合分离组氨酸标签(His-tag)蛋白质，特意去除螯合剂后尤其适合用于固定化金属亲和层析(IMAC)分离。

推荐用量：1 mL溶液抑制20g大肠杆菌细胞或10g杆状病毒感染草地夜蛾蛹卵巢细胞得到的100ml细胞裂解液中的蛋白酶活性。

特点和优势

专门为组氨酸标签蛋白纯化而配制。

靶向多种蛋白酶，具有全面的蛋白酶抑制活性。

不加螯合剂，适合IMAC应用。

方便的包装选择。

组分

AEBSF
乌苯美司
E-64
Pepstatin A
磷阿米酮

数量

推荐采用1mL抑制从20gEscherichia coli或10g杆状病毒感染 Spodoptera frugiperda蛹卵巢细胞提取的总体积100ml的蛋白酶。

该蛋白酶抑制剂混合物已针对组蛋白标记蛋白进行了优化和测试

制备说明

该产品在DMSO中是澄清溶液。 推荐1mL溶液可以抑制20gE. Coli细胞或10g细胞或10g 杆状病毒感染细胞得到的100ml细胞裂解液中的蛋白酶活性。

相关产品

产品编号
说明
价格

P8465

蛋白酶抑制剂混合物粉末, for use with bacterial cell extracts, lyophilized powder

P8340

Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

P8215

Protease Inhibitor Cocktail, for use with fungal and yeast extracts, DMSO solution

P2714

蛋白酶抑制剂混合物粉末, for general use, lyophilized powder

P9599

Protease Inhibitor Cocktail, for plant cell and tissue extracts, DMSO solution

P1860

Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution

S8820

SIGMAFAST™蛋白酶抑制剂片剂, For General Use

S8830

SIGMAFAST ™蛋白酶抑制剂混合物片剂，无EDTA, for use in purification of Histidine-tagged proteins

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

185.0 °F - closed cup

闪点(°C)

85 °C - closed cup

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000305631
0000305631
0000226095
0000226094
0000226094

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the difference between Protease Inhibitors P8849 and S8830?

    P8849 is a DMSO solution and S8830 is a water soluble tablet. P8849 formulation is considered proprietary but does contain AEBSF, Pepstatin A, Bestatin, E-64, and Phosphoramidon. The formulation of S8830 also contains Aprotinin and Pepstatin A and the exact formulation is given on the product information sheet (under Documents, above).

  6. Can Product No. P8849, Protease Inhibitor Cocktail, be used with with HIS-Select™ products or any other immobilized metal affinity chromatography (IMAC) products?

    This product does not contain EDTA and can be used with sample processing by immobilized metal affinity chromatography (IMAC) suchas used for the purification of histidine-tagged proteins..

  7. How much of Product No. P8849, Protease Inhibitor Cocktail, should be added to the cell lysate?

    A 1:100 dilution is the recommended concentration.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Z Kurowska et al.
Neuroscience, 256, 456-466 (2013-10-26)
Nogo-A is a transmembrane protein originally discovered in myelin, produced by postnatal CNS oligodendrocytes. Nogo-A induces growth cone collapse and inhibition of axonal growth in the injured adult CNS. In the intact CNS, Nogo-A functions as a negative regulator of
C Rivier et al.
The EMBO journal, 20(7), 1765-1773 (2001-04-04)
In Chlamydomonas reinhardtii, the psaA mRNA is assembled by a process involving trans-splicing of separate transcripts, encoded at three separate loci of the chloroplast genome. At least 14 nuclear loci and one chloroplast gene, tscA, are needed for this process.
Angelo Gallo et al.
Biomolecular NMR assignments, 15(1), 85-89 (2020-11-24)
Among the proteins encoded by the SARS-CoV-2 RNA, nsP3 (non-structural Protein3) is the largest multi-domain protein. Its role is multifaceted and important for the viral life cycle. Nonetheless, regarding the specific role of each domain there are many aspects of
E Boudreau et al.
The EMBO journal, 19(13), 3366-3376 (2000-07-06)
The psbD mRNA, which encodes the D2 reaction center polypeptide of photosystem II, is one of the most abundant chloroplast mRNAs. We have used genomic complementation to isolate the nuclear Nac2 gene, which is required for the stable accumulation of
Shuji Kaneko et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 22(1), 82-92 (2002-01-05)
The physical interaction between the presynaptic vesicle release complex and the large cytoplasmic region linking domains II and III of N-type (Ca(v)2.2) calcium channel alpha(1)B subunits is considered to be of fundamental importance for efficient neurotransmission. By PCR analysis of
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