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安全信息

NXTRACT

Sigma-Aldrich

NuCLEAR™提取试剂盒

For mammalian tissue or cultured cells

别名:

Nuclear Isolation Kit

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About This Item

UNSPSC代码:
41105517
NACRES:
NA.56

质量水平

300

用途

 kit sufficient for 10 extractions (1 ml packed cell volume)
 kit sufficient for 100 extractions (100 μl packed cell volume)

技术

protein extraction: suitable
western blot: suitable

运输

dry ice

储存温度

−20°C

相关类别

一般描述

其中的核内蛋白提取方法步骤可使细胞在低渗缓冲液中膨胀。随后细胞破碎，细胞质成分去除，核内蛋白质在高盐缓冲液条件下释放出来。

应用

NXTRACT试剂盒用于研究盐对降压性盐皮质激素治疗小鼠心脏差异基因表达和冠状动脉病变的影响。它还用于测试穿心莲内酯在治疗子宫内膜异位症方面的潜在作用。

其他说明

此试剂盒包含一套从哺乳动物组织或培养细胞制备细胞核和细胞质蛋白提取物的完整系统。所有提取所需的试剂均包含在内。
通过详细技术公告中的大量操作流程，可实现特定应用最适合的选择。 例如，可选择含去垢剂和不含去垢剂的核蛋白提取程序，或者适用于大多数细胞类型的标准低渗裂解缓冲液及适用于脆弱细胞的等渗裂解缓冲液。 此外，该试剂盒还提供了通过稀释缓冲液从核提取物中减少盐分的操作流程。 NuCLEAR可灵活满足您的理想蛋白提取需求。 可在2小时内制出提取物，且由于核提取物与细胞质提取物之间几乎不存在交叉污染，因此纯度很高。

联系

用于免疫检测的推荐抗体L1293, N2662, AMAB90549

法律信息

NuCLEAR is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • 3× Dilution and Equilibration Buffer 90 mL
  • P8340Protease Inhibitor Cocktail 1 mL化学品安全说明书

象形图

Corrosion
GHS05

警示用语：

Danger

危险声明

H290,H314

危险分类

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

储存分类代码

8A - Combustible corrosive hazardous materials

闪点(°F)

188.6 °F - closed cup

闪点(°C)

87 °C - closed cup

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000149724
0000149724
0000114896
059M4108V
11181117

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. What are the options for lysis of my cells to make extracts when using Product NXTRACT, CelLytic™ NuCLEAR™ Extraction Kit?

    The use of detergent will lead to maximum protein yield. However we find that the hypotonic lysis is extremely effective and in addition for very fragile cells we offer an isotonic lysis option.

  3. Can Product NXTRACT, CelLytic™ NuCLEAR™ Extraction Kit, be used to make nuclear protein extracts for EMSAs/DNA binding studies?

    Several references describe the use of the N-XTRACT kit in sample preparation for EMSA work.1. Xie, J., et al., β2-Microglobulin as a negative regulator of the immune system: high concentrations of the protein inhibit in vitro generation of functional dendritic cells. Blood, 101(10), 4005-4012 (2003).2. Marambaud, P., et al., A CBP Binding Transcriptional Repressor Produced by the PS1/ε-Cleavage of N-Cadherin Is Inhibitedby PS1 FAD Mutations. Cell, 114, 635-645 (2003).3. Chen, G., et al., Phosphorylated FADD induces NF-κB, perturbs cell cycle, and is associated with poor outcome in lung adenocarcinomas. Proc. Nat. Acad. Sci. USA, 102(35), 12507-12512 (2005).

  4. Is Product NXTRACT, CelLytic™ NuCLEAR™ Extraction Kit, suitable for use on tissues? 

    We have used the kit with several tissues: Mouse lung and brain, Rat liver and Rabbit muscle. For these tissues we usually used the kit with 100 mg tissue. After extraction with 140 μl Extraction buffer, we obtained an average yield of protein concentration as follows: 3.2 mg/ml for brain, 4.1 mg/ml for lung, 3.3 mg/ml for muscle and 10 mg/ml for liver.

  5. When using Product NXTRACT, CelLytic™ NuCLEAR™ Extraction Kit, what can I use for the mechanical lysis of my cells?

    We recommend the use of a glass tissue homogenizer, with a type B pestle, or alternatively a syringe with a narrow-gauge (No. 27) hypodermic needle may be used.

  6. How "pure" is the nuclear extraction using Product NXTRACT, CelLytic™ NuCLEAR™ Extraction Kit? 

    It is important to keep in mind that this kit will provide a crude nuclear fraction.  Depending on the care taken at the step where the nuclei are pelleted and the supernatant containing the cytoplasmic fraction is removed, we have found that the cytoplasmic contamination of the nuclear pellet may be a few percent.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

F Guidez et al.
Molecular and cellular biology, 18(7), 3851-3861 (1998-06-25)
Granulocyte-macrophage colony-stimulating factor (GM-CSF) and macrophage colony-stimulating factor (M-CSF) independently stimulate the proliferation and differentiation of macrophages from bone marrow progenitor cells. Although the GM-CSF and M-CSF receptors are unrelated, both couple to Ras-dependent signal transduction pathways, suggesting that these
Fangyuan Dong et al.
EBioMedicine, 39, 472-483 (2018-12-12)
Accumulating evidence has revealed the pivotal role of epigenetic regulation in the pathogenesis of liver disease. However, the epigenetic mechanism that accounts for hepatic stellate cells (HSCs) activation in liver fibrosis remains largely unknown. Primary HSCs were used to screen
Isolation of intact nuclei for nuclear extract preparation from a fragile B-lymphocyte cell line.
R B Dyer et al.
BioTechniques, 19(2), 192-195 (1995-08-01)
Qing Wang et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 302(9), R1025-R1033 (2012-03-10)
We previously reported that excess of deoxycorticosterone-acetate (DOCA)/salt-induced cardiac hypertrophy in the absence of hypertension in one-renin gene mice. This model allows us to study molecular mechanisms of high-salt intake in the development of cardiovascular remodeling, independently of blood pressure
K A Lee et al.
Gene analysis techniques, 5(2), 22-31 (1988-03-01)
A convenient and rapid method for preparing soluble extracts from the nuclei of as few as 3 x 10(7) mammalian cells (miniextract procedure) is described. By several criteria, miniextracts are comparable to nuclear extracts prepared from large numbers of cells
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