CN¥2,731.81
预计发货时间June 30, 2025详情
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用途
sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)
质量水平
保质期
1 yr at 2‑8 °C
包装
pkg of 1 kit
应用
cell analysis
异质活性
nuclease and protease, free
运输
wet ice
储存温度
2-8°C
1 of 4
此商品 | NUC201 | NXTRACT | EZFREAG57 |
|---|---|---|---|
| usage sufficient for 25 nuclei preparations (~1-10×107 cells/preparation) | usage sufficient for 15 nuclei preparations (~1-10×107 cells or 1g of tissue per preparation) | usage kit sufficient for 10 extractions (1 ml packed cell volume), kit sufficient for 100 extractions (100 μl packed cell volume) | usage - |
| shelf life 1 yr at 2‑8 °C | shelf life - | shelf life - | shelf life - |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging - | packaging - |
| application(s) cell analysis | application(s) cell analysis | application(s) - | application(s) food and beverages |
| foreign activity nuclease and protease, free | foreign activity nuclease and protease, free | foreign activity - | foreign activity - |
| shipped in wet ice | shipped in wet ice | shipped in dry ice | shipped in ambient |
一般描述
应用
生化/生理作用
其他说明
相关产品
储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Can Product NUC101, Nuclei Isolation Kit, be used to capture soluble nuclear proteins?
The kit was originally designed to produce nuclei that are competent for transcription run-on/run-off assays to measure transcription from pre-engaged polymerase complexes on the nuclear DNA. It was not designed for the separation or isolation of soluble nuclear proteins. The researcher might be advised to try the NXTRACT nuclear extraction kit, which allows for the isolation of "intact" nuclei from other cellular components before the lysis of the nuclear membrane.
Can I use Product NUC101, Nuclei Isolation Kit, on frozen cells?
It is likely that the freezing and thawing processes will damage many of the cells and nuclei, which could lead to loss of nuclear components and/or contamination of the nuclei with cytoplasmic or other cellular components.
Should I use trypsin to harvest my adherent cells before using Product NUC101, Nuclei Isolation Kit?
In general, this is not advised. We have never tried detaching the cells first with trypsin. We would not recommend it, because residual trypsin could clip nuclear proteins and cause problems in downstream applications.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
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