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I8896

Sigma-Aldrich

IGEPAL® CA-630

for molecular biology

别名:

辛基苯氧基聚（乙烯氧基）乙醇，支链, 聚氧乙烯辛烷基苯酚醚，分支, 辛基苯基-聚乙烯二醇

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About This Item

线性分子式:
(C2H4O)nC14H22O
CAS号:
9002-93-1
UNSPSC代码:
12352104
NACRES:
NA.31

等级

for molecular biology

质量水平

200

描述

non-ionic

表单

liquid

技术

IR spectroscopy: suitable

CMC

0.08 mM (20-25°C)

HLB

13

异质活性

DNase and RNase, none detected

SMILES字符串

O(CCO)c1ccc(cc1)C(CC(C)(C)C)(C)C

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

InChI key

JYCQQPHGFMYQCF-UHFFFAOYSA-N

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相关类别

一般描述

IGEPAL® CA-630是一种非离子型、非变性去污剂，适用于膜蛋白复合物的溶解、分离和纯化。它与Nonidet P-40在化学上无法区分。

应用

IGEPAL®CA-630已用于
  • 在小鼠肌肉匀浆的western blotting中作为匀浆缓冲液中的中性洗涤剂
  • 研究E8.5-E10.5胚胎中lacZ表达的X-gal染色
  • 制备可用于RT-qPCR的细胞裂解物的细胞裂解（CL）缓冲液

其他说明

非离子型、非变性洗涤剂

质量

在化学性质方面，不能与已经退市的 Nonidet P-40 区分开。

注意

在贮存过程中可能产生混浊或沉淀物。 可在加热至时40°C变为澄清液体。

法律信息

IGEPAL is a registered trademark of Solvay

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

483.8 °F - closed cup

闪点(°C)

251 °C - closed cup

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
MKCW7585
MKCV4219
MKCR5566
MKCR3234
MKCS4114

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. I am looking for Nonidet 40, but I am unable to find it. Do you sell this product? If not, can you suggest a suitable alternate product?

    Nonidet 40 or NP40 is no longer commercially available. The Igepal CA-630 (Product No. I8896), is the best substitute product that we can recommend.

  7. Are the 2 products, Igepal CA-630 (Product No. I8896) and Nonidet 40 the same? How do you use this product?

    Igepal CA-630 is chemically indistinguishable to NP40. This non-denaturing detergent is used the same way as that of NP40.

  8. In what form does Product No. I8896, IGEPAL® CA-630, arrive?

    It is a 100% concentrated liquid product. It is not diluted. Please check the Specification sheet available on our product detail page.

  9. How do we solubilize Product No. I8896, IGEPAL® CA-630?

    The product I8896 is miscible in all proportions with water.

  10. What is the formula weight of Product No. I8896, IGEPAL® CA-630?

    The formula weight of this product is 603.

  11. What is the CMC (critical micellar concentration) value of Product No. I8896, IGEPAL® CA-630?

    The CMC value of this product is 0.083 mM.

  12. What is the viscosity of Product No. I8896, IGEPAL® CA-630?

    The viscosity is determined for each lot and the information is available on the lot- specific Certificate of Analysis.

  13. What does "Molecular Biology Grade" mean?

    Molecular Biology Grade means that this product must meet the DNase, RNase, and Nickase specification on the Certificate of Analysis for each lot of the product.  

  14. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Joseph A Roche et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 59(11), 964-975 (2011-11-02)
Mutations in the DYSF gene that severely reduce the levels of the protein dysferlin are implicated in muscle-wasting syndromes known as dysferlinopathies. Although studies of its function in skeletal muscle have focused on its potential role in repairing the plasma
Bojana Lucic et al.
Nature communications, 10(1), 4059-4059 (2019-09-08)
HIV-1 recurrently targets active genes and integrates in the proximity of the nuclear pore compartment in CD4+ T cells. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained
Amina Abbadi et al.
The Journal of biological chemistry, 291(3), 1448-1455 (2015-11-26)
Many cells, including murine airway epithelial cells, respond to a variety of inflammatory stimuli by synthesizing leukocyte-adhesive hyaluronan (HA) cables that remain attached to their cell surfaces. This study shows that air-liquid interface cultures of murine airway epithelial cells (AECs)
Per Wasteson et al.
Development (Cambridge, England), 135(10), 1823-1832 (2008-04-18)
Aortic smooth muscle cells (SMCs) have been proposed to derive from lateral plate mesoderm. It has further been suggested that induction of SMC differentiation is confined to the ventral side of the aorta, and that SMCs later migrate to the
Kenneth Shatzkes et al.
Scientific reports, 4, 4659-4659 (2014-04-12)
Sample nucleic acid purification can often be rate-limiting for conventional quantitative PCR (qPCR) workflows. We recently developed high-throughput virus microneutralization assays using an endpoint assessment approach based on reverse transcription qPCR (RT-qPCR). The need for cumbersome RNA purification is circumvented
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实验方案

Protocol for Anti Ago-RNA Immunoprecipitation from Mammalian Cells Using the RIP Kit

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

RIP试剂盒的哺乳动物细胞Ago抗体-RNA免疫沉淀实验方案

使用RIP试剂盒进行哺乳动物细胞Ago抗体-RNA免疫沉淀的实验方案和流程

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

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Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

蛋白质Pull-Down技术

利用亲和力、GST pull-down、TAP 和共免疫沉淀方法，通过Pull-Down研究体外蛋白质互作。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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