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用途
sufficient for 15 nuclei preparations (~1-10×107 cells or 1g of tissue per preparation)
质量水平
包装
pkg of 1 kit
储存条件
dry at room temperature
应用
cell analysis
异质活性
nuclease and protease, free
运输
wet ice
储存温度
2-8°C
应用
用于从细胞系和实体组织制备高纯度胞核和脆性胞核。
生化/生理作用
该实验方案整合了垫层蔗糖密度梯度离心法，可以保护细胞核，免除细胞质污染。 关于特定细胞类型适宜的蔗糖浓度，可由用户根据经验确定。蔗糖浓度和蔗糖垫层缓冲液可让用户根据需要灵活修改蔗糖垫层密度。可采用常用细胞系（Jurkat、HFN7.1、COS7、HEK293和MDCK）和组织（脾脏和肝脏）获得高得率。该制备适用于许多细胞生物学应用，例如：为染色质、基因组DNA、组蛋白和核RNA/RNP提供细胞核来源，为体外凋亡检测、功能性研究（如细胞转录状态检查）制备细胞核。
该实验方案整合了垫层蔗糖密度梯度离心法，可以保护细胞核，免除细胞质污染。可采用常用细胞系（Jurkat、HFN7.1、COS7、HEK293和MDCK）和组织（脾脏和肝脏）获得高得率。该制备适用于许多细胞生物学应用，例如：为染色质、基因组DNA、组蛋白和核RNA/RNP提供细胞核来源，为体外凋亡检测、功能性研究（如细胞转录状态检查）制备细胞核。
仅试剂盒组分
产品编号
说明
- Nuclei PURE Lysis Buffer 180 mL
相关产品
产品编号
说明
价格
警示用语：
Danger
危险声明
危险分类
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Analysis of nuclear RNA, Chapter 14.
Robert E. Farrell, Jr., ed.
RNA Methodologies: A Laboratory Guide for Isolation and Characterization, 235-263 (1993)
Nuclei from rat liver: isolation method that combines purity with high yield.
G Blobel et al.
Science (New York, N.Y.), 154(3757), 1662-1665 (1966-12-30)
Michaela Patterson et al.
Nature genetics, 49(9), 1346-1353 (2017-08-08)
Adult mammalian cardiomyocyte regeneration after injury is thought to be minimal. Mononuclear diploid cardiomyocytes (MNDCMs), a relatively small subpopulation in the adult heart, may account for the observed degree of regeneration, but this has not been tested. We surveyed 120
Baochen Shi et al.
BMC genomics, 10, 92-92 (2009-02-27)
A major goal of post-genomics research is the integrated analysis of genes, regulatory elements and the chromatin architecture on a genome-wide scale. Mapping DNase I hypersensitive sites within the nuclear chromatin is a powerful and well-established method of identifying regulatory
Tyler G Ekins et al.
eLife, 9 (2020-11-06)
Type I lissencephaly is a neuronal migration disorder caused by haploinsuffiency of the PAFAH1B1 (mouse: Pafah1b1) gene and is characterized by brain malformation, developmental delays, and epilepsy. Here, we investigate the impact of Pafah1b1 mutation on the cellular migration, morphophysiology
商品
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
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