NUC201
细胞核分离试剂盒：Nuclei PURE Prep
sufficient for 15 nuclei preparations (~1-10×107 cells or 1g of tissue per preparation)
别名:
Sucrose centrifugation nuclei isolation
产品名称
细胞核分离试剂盒：Nuclei PURE Prep, sufficient for 15 nuclei preparations (~1-10×107 cells or 1g of tissue per preparation)
usage
sufficient for 15 nuclei preparations (~1-10×107 cells or 1g of tissue per preparation)
packaging
pkg of 1 kit
storage condition
dry at room temperature
application(s)
cell analysis
foreign activity
nuclease and protease, free
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Application
用于从细胞系和实体组织制备高纯度胞核和脆性胞核。
Biochem/physiol Actions
该实验方案整合了垫层蔗糖密度梯度离心法，可以保护细胞核，免除细胞质污染。 关于特定细胞类型适宜的蔗糖浓度，可由用户根据经验确定。蔗糖浓度和蔗糖垫层缓冲液可让用户根据需要灵活修改蔗糖垫层密度。可采用常用细胞系（Jurkat、HFN7.1、COS7、HEK293和MDCK）和组织（脾脏和肝脏）获得高得率。该制备适用于许多细胞生物学应用，例如：为染色质、基因组DNA、组蛋白和核RNA/RNP提供细胞核来源，为体外凋亡检测、功能性研究（如细胞转录状态检查）制备细胞核。
该实验方案整合了垫层蔗糖密度梯度离心法，可以保护细胞核，免除细胞质污染。可采用常用细胞系（Jurkat、HFN7.1、COS7、HEK293和MDCK）和组织（脾脏和肝脏）获得高得率。该制备适用于许多细胞生物学应用，例如：为染色质、基因组DNA、组蛋白和核RNA/RNP提供细胞核来源，为体外凋亡检测、功能性研究（如细胞转录状态检查）制备细胞核。
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Nuclei from rat liver: isolation method that combines purity with high yield.
G Blobel et al.
Science (New York, N.Y.), 154(3757), 1662-1665 (1966-12-30)
Analysis of nuclear RNA, Chapter 14.
Robert E. Farrell, Jr., ed.
RNA Methodologies: A Laboratory Guide for Isolation and Characterization, 235-263 (1993)
Tyler G Ekins et al.
eLife, 9 (2020-11-06)
Type I lissencephaly is a neuronal migration disorder caused by haploinsuffiency of the PAFAH1B1 (mouse: Pafah1b1) gene and is characterized by brain malformation, developmental delays, and epilepsy. Here, we investigate the impact of Pafah1b1 mutation on the cellular migration, morphophysiology
Transcription of RNA in isolated nuclei, Chapter 4.
Marzluff, W.F., and Huang, R.C.C. et al.
Transcription and Translation: A Practical Approach,, 89-129 (1984)
Sundong Lin et al.
Diabetes & metabolism journal, 44(1), 158-172 (2019-11-09)
Epithelial-to-mesenchymal transition (EMT) is required for renal fibrosis, which is a characteristic of diabetic nephropathy (DN). Our previous study demonstrated that fibroblast growth factor 21 (FGF21) prevented DN associated with the suppressing renal connective tissue growth factor expression, a key
商品
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
相关内容
Instructions
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