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Merck
CN
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文件

安全信息

12-309

Sigma-Aldrich

HeLa Nuclear Extract

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About This Item

UNSPSC代码:
41105330
eCl@ss:
32011807
NACRES:
NA.41

生物来源

human

质量水平

形式

liquid

制造商/商品名称

Upstate®

浓度

2.0 mg/mL

技术

western blot: suitable

运输

dry ice

一般描述

Nuclear extract prepared from human HeLa cells using a modified protocol of Dignam, et al., which contains a variety of DNA binding proteins and transcription factors.

细胞系描述

HeLa

质量

Routinely evaluated as a positive control for many of Upstates antibodies.

外形

RIPA sample buffer containing inhibitors. Frozen solution.

储存及稳定性

Stable for 6 months at -70°C from date of shipment.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

高风险级别生物产品-Merck

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Tabetha Sundin et al.
Biochimie, 94(12), 2639-2648 (2012-08-21)
Isoprenoids are recognized for their ability to suppress carcinogenic processes in vivo and in vitro. We previously established that the isoprenoid, perillyl alcohol, acted mechanistically on translation of specific proteins through modulation of mechanistic target of rapamycin (mTOR) signaling. Telomerase-the
J D Dignam et al.
Nucleic acids research, 11(5), 1475-1489 (1983-03-11)
We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major

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