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Merck
CN

CS0760

线粒体分离物染色试剂盒

1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

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关于此项目

UNSPSC Code:
12352207
EC Number:
217-896-6
NACRES:
NA.32

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产品名称

线粒体分离物染色试剂盒, 1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

usage

 kit sufficient for 1000 reactions (using 96 multiwell plates)
 kit sufficient for 50 reactions (in a 2 mL cuvette)

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

flow cytometry: suitable
protein staining: suitable

fluorescence

λex 490 nm; λem 590 nm (JC-1 dye)

application(s)

cell analysis
detection

detection method

fluorometric

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

相关类别

Analysis Note

本试剂盒用于检测线粒体的完整性，以及线粒体膜电位的消失事件。

Application

独立的线粒体染色试剂盒已被用于评估线粒体内膜的电位。

Biochem/physiol Actions

本试剂盒基于JC-1染料的线粒体染色法。在正常细胞中，JC-1染料能够聚集于线粒体基质中，形成红色的荧光聚集物。任何导致线粒体膜电位消失的事件（如细胞凋亡）都会导致JC-1染料在线粒体内无法聚集。可通过荧光计或荧光显微镜来检测这一染料。

Features and Benefits

  • 简单快捷的方法，可用于对线粒体分离产物进行染色和检测线粒体完整性
  • 包含检测线粒体内膜电化学电位改变所需的全部试剂
  • 包含能够穿透线粒体膜的抗菌性缬氨霉素，因此能够消除线粒体的电化学电位。缬氨霉素可用做对照试剂，来避免JC-1的聚集。

pictograms

Skull and crossbones
GHS06

signalword

Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 1 Oral

存储类别

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000275141
0000275141
11180552
108M4080V
038M4176V

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商品

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Mitochondrial Stress and ROS

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

凋亡分析检测

细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。

线粒体应激和ROS

氧化应激，在一部分上，是由多种细胞过程产生的活性氧介导，并由诸如酶清除剂或抗氧化剂调节剂等细胞抗氧化剂机制所控制的。自由基，如活性氧，会通过细胞引起细胞损伤。

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