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MIT1000

Sigma-Aldrich

线粒体/胞质溶胶分离试剂盒

This Mitochondria/Cytosol Fractionation Kit provides reagents for quick & efficient isolation of intact mitochondria from cultured cells.

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About This Item

UNSPSC代码:
12161503
eCl@ss:
32161000
NACRES:
NA.75

质量水平

种属反应性(根据同源性预测)

all

制造商/商品名称

Chemicon®

技术

cell culture | mammalian: suitable

应用

sample preparation

运输

dry ice

一般描述

线粒体,有时被描述为细胞的“发电厂”,是真核细胞中大部分能量产生发生的场所。大多数三磷酸腺苷(ATP)的合成发生在活细胞中发现的这些双膜细胞器中。线粒体产生ATP是通过呼吸过程完成的,呼吸过程使用氧气产生能量。这是利用食物能量制造ATP的一个非常高效的过程。除了提供细胞能量外,线粒体还参与一系列其他过程,例如信号传导、细胞分化、细胞死亡以及细胞周期和细胞生长的控制。线粒体还调节关键的细胞凋亡信号通路。细胞中线粒体的数量因生物体和组织类型而异。许多细胞只有几个线粒体,而其他细胞则可以包含数千个。

Millipore’s线粒体/胞质分离试剂盒提供了从培养细胞中快速有效地分离完整线粒体的试剂。该试剂盒可通过使用方便的台式微量离心机分离线粒体,并可用于将富集的线粒体级分(重膜级分)与胞质级分(轻膜级分)分离。这种分离对于通过蛋白质印迹或ELISA研究两个部分之间的细胞凋亡和信号通路是有用的。

该试剂盒包含足以分析50个样品的材料。

应用

研究类别
细胞凋亡 & 癌症

组分

等渗线粒体缓冲液(CS204255): 50 mL

线粒体裂解缓冲液(CS204257): 5 mL

蛋白酶抑制剂混合物(CS204253): 1 mL

抗Bcl2(05-729-25UG): 25 μg

抗GAPDH(CS204254): 25 μg

储存及稳定性

自接收之日起,所有组件应在-20°C下储存长达一年。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的目录或产品随附的其他公司文件中另有说明,否则我们的产品预期仅用于研究用途,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2

储存分类代码

10 - Combustible liquids


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