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MAK159

线粒体膜电位检测试剂盒（适用于酶标仪）

Name: 线粒体膜电位检测试剂盒（适用于酶标仪）
Brand: SIGMA

sufficient for 500 fluorometric tests (microplate readers)

别名:

JC-10检测, JC-10线粒体膜蛋白检测

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About This Item

UNSPSC代码:

12161503

NACRES:

NA.25

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由于电子传递链上酶的活动，线粒体在其膜上产生电位。[1]在细胞凋亡过程中，线粒体膜电位（MMP）的崩溃与线粒体通透性转换孔的开放一致，导致细胞色素c 释放到细胞质中，进而引发细胞凋亡级联反应的其他下游事件。[2][3]

应用

线粒体膜电位试剂盒已用于测量线粒体膜电位。[4][5]

适用性

本试剂盒适于检测哺乳动物细胞中的线粒体跨膜电势差(MMP)，并可用于在酶标仪上筛选凋亡抑制剂及激活剂等。

原理

该试剂盒利用 JC-10，一种优于JC-1的替代品，用于测定细胞中MMP的损失。尽管 JC-1在许多实验室中被广泛使用，但是当其在较高浓度下使用时，其水溶性差通常会导致水性缓冲液沉淀。在较高浓度下，JC-10 表现出比 JC-1 更高的水溶性。与JC-1 类似，JC-10是一种阳离子亲脂性染料，其浓缩并在具有极化线粒体膜的细胞线粒体中形成可逆的红色荧光 JC-10 聚集体（λ_ex = 540/λ_em = 590 nm）。在凋亡细胞中，MMP 崩溃导致 JC-10 无法保留在线粒体中，并使染料返回到单体绿色荧光形式（λ_ex = 490/λ_em = 525 nm）。该试剂盒可用于监测细胞凋亡和筛选凋亡抑制剂和激活剂。

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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MiR-195 dependent roles of mitofusin2 in the mitochondrial dysfunction of hippocampal neurons in SAMP8 mice.

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细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。

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此商品	567532U	567531U	567502U
567501U Discovery ^® HS F5 HPLC 色谱柱	567532U Discovery ^® HS F5 HPLC 色谱柱	567531U Discovery ^® HS F5 HPLC 色谱柱	567502U Discovery ^® HS F5 HPLC 色谱柱
separation technique reversed phase	separation technique reversed phase	separation technique reversed phase	separation technique reversed phase
matrix active group PFP (pentafluorophenyl) phase	matrix active group PFP (pentafluorophenyl) phase	matrix active group PFP (pentafluorophenyl) phase	matrix active group PFP (pentafluorophenyl) phase
matrix silica gel, high purity, spherical particle platform, fully porous particle	matrix silica gel, high purity, spherical particle platform, fully porous particle	matrix silica gel, high purity, spherical particle platform, fully porous particle	matrix silica gel, high purity, spherical particle platform, fully porous particle
agency suitable for USP L43	agency suitable for USP L43	agency suitable for USP L43	agency suitable for USP L43
technique(s) HPLC: suitable, LC/MS: suitable	technique(s) HPLC: suitable, LC/MS: suitable	technique(s) HPLC: suitable, LC/MS: suitable	technique(s) HPLC: suitable, LC/MS: suitable

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线粒体膜电位检测试剂盒（适用于酶标仪）

sufficient for 500 fluorometric tests (microplate readers)

About This Item

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