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用途
sufficient for 500 fluorometric tests (microplate readers)
检测方法
fluorometric
相关疾病
cancer
储存温度
−20°C
一般描述
由于电子传递链上酶的活动，线粒体在其膜上产生电位。在细胞凋亡过程中，线粒体膜电位（MMP）的崩溃与线粒体通透性转换孔的开放一致，导致细胞色素c 释放到细胞质中，进而引发细胞凋亡级联反应的其他下游事件。
应用
线粒体膜电位试剂盒已用于测量线粒体膜电位。
适用性
本试剂盒适于检测哺乳动物细胞中的线粒体跨膜电势差(MMP)，并可用于在酶标仪上筛选凋亡抑制剂及激活剂等。
原理
该试剂盒利用 JC-10，一种优于JC-1的替代品，用于测定细胞中MMP的损失。尽管 JC-1在许多实验室中被广泛使用，但是当其在较高浓度下使用时，其水溶性差通常会导致水性缓冲液沉淀。在较高浓度下，JC-10 表现出比 JC-1 更高的水溶性。与JC-1 类似，JC-10是一种阳离子亲脂性染料，其浓缩并在具有极化线粒体膜的细胞线粒体中形成可逆的红色荧光 JC-10 聚集体（λex = 540/λem = 590 nm）。在凋亡细胞中，MMP 崩溃导致 JC-10 无法保留在线粒体中，并使染料返回到单体绿色荧光形式（λex = 490/λem = 525 nm）。该试剂盒可用于监测细胞凋亡和筛选凋亡抑制剂和激活剂。
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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商品
细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。
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